Technical Resources

22340 - Medium 199, HEPES

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 50.0 0.6666667
L-Alanine 89.0 25.0 0.28089887
L-Arginine hydrochloride 211.0 70.0 0.33175355
L-Aspartic acid 133.0 30.0 0.22556391
L-Cysteine hydrochloride-H2O 176.0 0.1 5.681818E-4
L-Cystine 2HCl 240.0 26.0 0.108333334
L-Glutamic Acid 147.0 75.0 0.5102041
L-Glutamine 146.0 100.0 0.6849315
L-Histidine hydrochloride-H2O 210.0 21.88 0.10419047
L-Hydroxyproline 131.0 10.0 0.07633588
L-Isoleucine 131.0 40.0 0.3053435
L-Leucine 131.0 60.0 0.45801526
L-Lysine hydrochloride 183.0 70.0 0.38251367
L-Methionine 149.0 15.0 0.10067114
L-Phenylalanine 165.0 25.0 0.15151516
L-Proline 115.0 40.0 0.3478261
L-Serine 105.0 25.0 0.23809524
L-Threonine 119.0 30.0 0.25210086
L-Tryptophan 204.0 10.0 0.04901961
L-Tyrosine disodium salt dihydrate 261.0 58.0 0.22222222
L-Valine 117.0 25.0 0.21367522
Vitamins
Alpha-tocopherol Phosphate 702.0 0.01 1.42450135E-5
Ascorbic Acid 176.0 0.05 2.840909E-4
Biotin 244.0 0.01 4.0983607E-5
Choline chloride 140.0 0.5 0.0035714286
D-Calcium pantothenate 477.0 0.01 2.096436E-5
Folic Acid 441.0 0.01 2.2675737E-5
Menadione (Vitamin K3) 172.0 0.01 5.8139532E-5
Niacinamide 122.0 0.025 2.0491803E-4
Nicotinic acid (Niacin) 123.0 0.025 2.0325204E-4
Para-Aminobenzoic Acid 137.0 0.05 3.6496352E-4
Pyridoxal hydrochloride 204.0 0.025 1.2254903E-4
Pyridoxine hydrochloride 206.0 0.025 1.21359226E-4
Riboflavin 376.0 0.01 2.6595744E-5
Thiamine hydrochloride 337.0 0.01 2.967359E-5
Vitamin A (acetate) 328.0 0.1 3.0487805E-4
Vitamin D2 (Calciferol) 397.0 0.1 2.5188917E-4
i-Inositol 180.0 0.05 2.7777778E-4
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 200.0 1.8018018
Ferric nitrate (Fe(NO3)-9H2O) 404.0 0.7 0.0017326733
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 97.67 0.8139166
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2200.0 26.190475
Sodium Chloride (NaCl) 58.0 6100.0 105.17242
Sodium Phosphate monobasic (NaH2PO4) anhydrous 138.0 140.0 1.0144928
Other Components
2-deoxy-D-ribose 134.0 0.5 0.0037313432
Adenine sulfate 404.0 10.0 0.024752475
Adenosine 5'-phosphate 347.0 0.2 5.763689E-4
Adenosine 5'-triphosphate 605.0 1.0 0.0016528926
Cholesterol 387.0 0.2 5.1679584E-4
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
Glutathione (reduced) 307.0 0.05 1.6286645E-4
Guanine hydrochloride 188.0 0.3 0.0015957447
HEPES 238.0 5960.0 25.042017
Hypoxanthine Na 136.0 0.4 0.0029411765
Phenol Red 376.4 20.0 0.053134963
Ribose 150.0 0.5 0.0033333334
Sodium Acetate 82.0 50.0 0.6097561
Thymine 126.0 0.3 0.0023809525
Tween 80® 20.0 Infinity
Uracil 112.0 0.3 0.0026785715
Xanthine-Na 152.0 0.34 0.0022368422

Reference:

1. Morgan, J.F. and Campbell, M.E. (1955) J. Natl. Cancer Inst., 16:557.

2. Morgan, J.F., Morton, H.J. and Parker R.C. (1950) Proc. Soc. Exp. 73:1.