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31153 - Medium 199, low sodium bicarbonate

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 50.0 0.6666667
L-Alanine 89.0 25.0 0.28089887
L-Arginine hydrochloride 211.0 70.0 0.33175355
L-Aspartic acid 133.0 30.0 0.22556391
L-Cysteine hydrochloride-H2O 176.0 0.1 5.681818E-4
L-Cystine 2HCl 240.0 26.0 0.108333334
L-Glutamic Acid 147.0 66.8 0.4544218
L-Glutamine 146.0 100.0 0.6849315
L-Histidine hydrochloride-H2O 210.0 21.88 0.10419047
L-Hydroxyproline 131.0 10.0 0.07633588
L-Isoleucine 131.0 40.0 0.3053435
L-Leucine 131.0 60.0 0.45801526
L-Lysine hydrochloride 183.0 70.0 0.38251367
L-Methionine 149.0 15.0 0.10067114
L-Phenylalanine 165.0 25.0 0.15151516
L-Proline 115.0 40.0 0.3478261
L-Serine 105.0 25.0 0.23809524
L-Threonine 119.0 30.0 0.25210086
L-Tryptophan 204.0 10.0 0.04901961
L-Tyrosine disodium salt dihydrate 261.0 58.0 0.22222222
L-Valine 117.0 25.0 0.21367522
Vitamins
Ascorbic Acid 176.0 0.05 2.840909E-4
Biotin 244.0 0.01 4.0983607E-5
Choline chloride 140.0 0.5 0.0035714286
D-Calcium pantothenate 477.0 0.01 2.096436E-5
Folic Acid 441.0 0.01 2.2675737E-5
Menadione (Vitamin K3) 172.0 0.01 5.8139532E-5
Niacinamide 122.0 0.025 2.0491803E-4
Nicotinic acid (Niacin) 123.0 0.025 2.0325204E-4
Para-Aminobenzoic Acid 137.0 0.05 3.6496352E-4
Pyridoxal hydrochloride 204.0 0.025 1.2254903E-4
Pyridoxine hydrochloride 206.0 0.025 1.21359226E-4
Riboflavin 376.0 0.01 2.6595744E-5
Thiamine hydrochloride 337.0 0.01 2.967359E-5
Vitamin A (acetate) 328.0 0.1 3.0487805E-4
Vitamin D2 (Calciferol) 397.0 0.1 2.5188917E-4
alpha Tocopherol phos. Na salt 554.7 0.01 1.8027762E-5
i-Inositol 180.0 0.05 2.7777778E-4
Inorganic Salts
Calcium Chloride (CaCl2-2H2O) 147.0 264.0 1.7959183
Ferric nitrate (Fe(NO3)-9H2O) 404.0 0.7 0.0017326733
Magnesium Sulfate (MgSO4-7H2O) 246.0 200.0 0.8130081
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 1250.0 14.880953
Sodium Chloride (NaCl) 58.0 6800.0 117.24138
Sodium Phosphate monobasic (NaH2PO4-2H2O) 156.0 158.0 1.0128205
Other Components
2-deoxy-D-ribose 134.0 0.5 0.0037313432
Adenine sulfate 404.0 10.0 0.024752475
Adenosine 5'-phosphate 347.0 0.2 5.763689E-4
Adenosine 5'-triphosphate 605.0 1.0 0.0016528926
Cholesterol 387.0 0.2 5.1679584E-4
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
Glutathione (reduced) 307.0 0.05 1.6286645E-4
Guanine hydrochloride 188.0 0.3 0.0015957447
Hypoxanthine 136.0 0.3 0.0022058825
Phenol Red 376.4 20.0 0.053134963
Ribose 150.0 0.5 0.0033333334
Sodium acetate-3H2O 136.0 83.0 0.6102941
Thymine 126.0 0.3 0.0023809525
Tween 80® 20.0 Infinity
Uracil 112.0 0.3 0.0026785715
Xanthine 174.0 0.344 0.0019770116

Reference:

1. Morgan, J.F. and Campbell, M.E. (1955) J. Natl. Cancer Inst., 16:557.

2. Morgan, J.F., Morton, H.J. and Parker R.C. (1950) Proc. Soc. Exp. Biol. Med., 73:1.

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