Technical Resources

A12176 - Williams' Medium E, no Phenol Red

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 50.0 0.6666667
L-Alanine 89.0 90.0 1.011236
L-Arginine 174.0 50.0 0.28735632
L-Asparagine-H2O 150.0 20.0 0.13333334
L-Aspartic acid 133.0 30.0 0.22556391
L-Cysteine 121.0 40.0 0.3305785
L-Cystine 2HCl 313.0 26.07 0.08329073
L-Glutamic Acid 147.0 50.0 0.34013605
L-Histidine 155.0 15.0 0.09677419
L-Isoleucine 131.0 50.0 0.3816794
L-Leucine 131.0 75.0 0.57251906
L-Lysine hydrochloride 183.0 87.46 0.47792348
L-Methionine 149.0 15.0 0.10067114
L-Phenylalanine 165.0 25.0 0.15151516
L-Proline 115.0 30.0 0.26086956
L-Serine 105.0 10.0 0.0952381
L-Threonine 119.0 40.0 0.33613446
L-Tryptophan 204.0 10.0 0.04901961
L-Tyrosine disodium salt dihydrate 261.0 50.65 0.19406131
L-Valine 117.0 50.0 0.42735043
Vitamins
Ascorbic Acid 176.0 2.0 0.011363637
Biotin 244.0 0.5 0.0020491802
Choline chloride 140.0 1.5 0.010714286
D-Calcium pantothenate 477.0 1.0 0.002096436
Ergocalciferol 397.0 0.1 2.5188917E-4
Folic Acid 441.0 1.0 0.0022675737
Menadione sodium bisulfate 276.0 0.01 3.6231882E-5
Niacinamide 122.0 1.0 0.008196721
Pyridoxal hydrochloride 204.0 1.0 0.004901961
Riboflavin 376.0 0.1 2.6595744E-4
Thiamine hydrochloride 337.0 1.0 0.002967359
Vitamin A (acetate) 328.0 0.1 3.0487805E-4
Vitamin B12 1355.0 0.2 1.4760147E-4
alpha Tocopherol phos. Na salt 554.7 0.01 1.8027762E-5
i-Inositol 180.0 2.0 0.011111111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 200.0 1.8018018
Cupric sulfate (CuSO4-5H2O) 250.0 1.0E-4 3.9999998E-7
Ferric sulfate (FeSO4-7H2O) 278.0 1.0E-4 3.5971223E-7
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 97.67 0.8139166
Manganese Sulfate (MnSO4-H20) 169.0 1.0E-4 5.9171595E-7
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2200.0 26.190475
Sodium Chloride (NaCl) 58.0 6800.0 117.24138
Sodium Phosphate monobasic (NaH2PO4) anhydrous 138.0 140.0 1.0144928
Zinc sulfate (ZnSO4-7H2O) 288.0 2.0E-4 6.9444445E-7
Other Components
D-Glucose (Dextrose) 180.0 2000.0 11.111111
Glutathione (reduced) 307.0 0.05 1.6286645E-4
Methyl linoleate 295.0 0.03 1.0169491E-4
Sodium Pyruvate 110.0 25.0 0.22727273

Reference:

1. Williams, G.M. and Gunn, J.M. (1974) Long term cell culture of adult rat liver epithelial cells. Exp. Cell Res., 89:139.

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