Technical Resources

21475 - Fischer’s Medium

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
L-Arginine hydrochloride 211.0 18.6 0.08815166
L-Asparagine 132.0 10.0 0.07575758
L-Cystine 2HCl 313.0 26.2 0.08370607
L-Glutamine 146.0 204.0 1.3972603
L-Histidine hydrochloride-H2O 210.0 66.0 0.31428573
L-Isoleucine 131.0 75.0 0.57251906
L-Leucine 131.0 30.0 0.22900763
L-Lysine hydrochloride 183.0 50.0 0.27322406
L-Methionine 149.0 100.0 0.67114097
L-Phenylalanine 165.0 60.0 0.36363637
L-Serine 105.0 15.0 0.14285715
L-Threonine 119.0 30.0 0.25210086
L-Tryptophan 204.0 10.0 0.04901961
L-Tyrosine disodium salt dihydrate 261.0 86.5 0.33141762
L-Valine 117.0 70.0 0.5982906
L-Valine 117.0 70.0 0.5982906
Vitamins
Choline chloride 140.0 1.5 0.010714286
D-Calcium pantothenate 477.0 0.5 0.001048218
Folic Acid 441.0 10.0 0.022675738
Niacinamide 122.0 0.5 0.0040983604
Pyridoxal hydrochloride 204.0 0.5 0.0024509805
Riboflavin 376.0 0.5 0.0013297872
Thiamine hydrochloride 337.0 1.0 0.002967359
d-Biotin 244.0 0.01 4.0983607E-5
i-Inositol 180.0 1.5 0.008333334
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 68.7 0.6189189
Magnesium Chloride (anhydrous) 95.0 47.0 0.49473685
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 1125.0 13.392858
Sodium Chloride (NaCl) 58.0 8000.0 137.93103
Sodium Phosphate dibasic (Na2HPO4) anhydrous 268.0 59.9 0.22350746
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 69.0 0.5
Other Components
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
Phenol Red 376.4 5.0 0.013283741

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