Technical Resources

12400 - DMEM/F-12, powder, HEPES

  • Catalog Number(s)

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 18.75 0.25
L-Alanine 89.0 4.45 0.049999997
L-Arginine hydrochloride 211.0 147.5 0.69905216
L-Asparagine-H2O 150.0 7.5 0.05
L-Aspartic acid 133.0 6.65 0.05
L-Cysteine hydrochloride-H2O 176.0 17.56 0.09977272
L-Cystine 2HCl 313.0 31.29 0.09996805
L-Glutamic Acid 147.0 7.35 0.05
L-Glutamine 146.0 365.0 2.5
L-Histidine hydrochloride-H2O 210.0 31.48 0.14990476
L-Isoleucine 131.0 54.47 0.41580153
L-Leucine 131.0 59.05 0.45076334
L-Lysine hydrochloride 183.0 91.25 0.4986339
L-Methionine 149.0 17.24 0.11570469
L-Phenylalanine 165.0 35.48 0.2150303
L-Proline 115.0 17.25 0.15
L-Serine 105.0 26.25 0.25
L-Threonine 119.0 53.45 0.44915968
L-Tryptophan 204.0 9.02 0.04421569
L-Tyrosine disodium salt dihydrate 261.0 55.79 0.21375479
L-Valine 117.0 52.85 0.4517094
Vitamins
Biotin 244.0 0.0035 1.4344263E-5
Choline chloride 140.0 8.98 0.06414285
D-Calcium pantothenate 477.0 2.24 0.0046960167
Folic Acid 441.0 2.65 0.0060090707
Niacinamide 122.0 2.02 0.016557377
Pyridoxine hydrochloride 206.0 2.031 0.009859223
Riboflavin 376.0 0.219 5.824468E-4
Thiamine hydrochloride 337.0 2.17 0.0064391694
Vitamin B12 1355.0 0.68 5.0184503E-4
i-Inositol 180.0 12.6 0.07
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 116.6 1.0504504
Cupric sulfate (CuSO4-5H2O) 250.0 0.0013 5.2E-6
Ferric Nitrate (Fe(NO3)3"9H2O) 404.0 0.05 1.2376238E-4
Ferric sulfate (FeSO4-7H2O) 278.0 0.417 0.0015
Magnesium Chloride (anhydrous) 95.0 28.64 0.30147368
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 48.84 0.407
Potassium Chloride (KCl) 75.0 311.8 4.1573334
Sodium Chloride (NaCl) 58.0 6995.5 120.61207
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142.0 71.02 0.50014085
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 62.5 0.45289856
Zinc sulfate (ZnSO4-7H2O) 288.0 0.432 0.0015
Other Components
D-Glucose (Dextrose) 180.0 3151.0 17.505556
HEPES 238.0 3574.5 15.018908
Hypoxanthine Na 159.0 2.39 0.015031448
Linoleic Acid 280.0 0.042 1.4999999E-4
Lipoic Acid 206.0 0.105 5.097087E-4
Phenol Red 376.4 8.1 0.021519661
Putrescine 2HCl 161.0 0.081 5.031056E-4
Sodium Pyruvate 55.0 Infinity
Thymidine 242.0 0.365 0.0015082645

Reference:

1. Dulbecco, R. and Freeman, G., (1959) Plaque formation by the polyoma virus. Virology 8:396.

2. Ham, R.G., (1965) Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.

3. Morton, H.J. (1970) A survey of commercially available tissue culture media. In Vitro 6(2):89.

4. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:185.

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