Technical Resources

11550 - Ham's F-10 Nutrient Mix

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 7.5 0.1
L-Alanine 89.0 9.0 0.101123594
L-Arginine hydrochloride 211.0 211.0 1.0
L-Asparagine-H2O 150.0 15.0 0.1
L-Aspartic acid 133.0 13.0 0.09774436
L-Cysteine 121.0 25.0 0.20661157
L-Glutamic Acid 147.0 14.7 0.1
L-Glutamine 146.0 146.0 1.0
L-Histidine hydrochloride-H2O 210.0 23.0 0.10952381
L-Isoleucine 131.0 2.6 0.019847328
L-Leucine 131.0 13.0 0.099236645
L-Lysine hydrochloride 183.0 29.0 0.15846995
L-Methionine 149.0 4.5 0.030201342
L-Phenylalanine 165.0 5.0 0.030303031
L-Proline 115.0 11.5 0.1
L-Serine 105.0 10.5 0.1
L-Threonine 119.0 3.6 0.0302521
L-Tryptophan 204.0 0.6 0.0029411765
L-Tyrosine disodium salt dihydrate 261.0 2.62 0.010038313
L-Valine 117.0 3.5 0.02991453
Vitamins
Biotin 244.0 0.024 9.836066E-5
Choline chloride 140.0 0.7 0.0050
D-Calcium pantothenate 477.0 0.7 0.0014675052
Folic Acid 441.0 1.3 0.0029478457
Niacinamide 122.0 0.6 0.004918033
Pyridoxine hydrochloride 206.0 0.2 9.708738E-4
Riboflavin 376.0 0.4 0.0010638298
Thiamine hydrochloride 337.0 1.0 0.002967359
Vitamin B12 1355.0 1.4 0.0010332103
i-Inositol 180.0 0.5 0.0027777778
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 33.3 0.29999998
Cupric sulfate (CuSO4-5H2O) 250.0 0.0025 1.0E-5
Ferric sulfate (FeSO4-7H2O) 278.0 0.834 0.0030
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 74.62 0.6218334
Potassium Chloride (KCl) 75.0 285.0 3.8
Potassium Phosphate monobasic (KH2PO4) 136.0 83.0 0.6102941
Sodium Bicarbonate (NaHCO3) 84.0 1200.0 14.285714
Sodium Chloride (NaCl) 58.0 7400.0 127.586205
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142.0 153.7 1.0823944
Zinc sulfate (ZnSO4-7H2O) 288.0 0.03 1.0416666E-4
Other Components
D-Glucose (Dextrose) 180.0 1100.0 6.111111
Hypoxanthine Na 159.0 4.7 0.029559746
Lipoic Acid 206.0 0.2 9.708738E-4
Phenol Red 376.4 1.2 0.0031880978
Sodium Pyruvate 110.0 110.0 1.0
Thymidine 242.0 0.7 0.002892562

Reference:

1. Ham, R.G., (1963) An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp. Cell Res., 29:515.

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