Technical Resources

21127 - Ham's F-12K (Kaighn's)

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 15.0 0.2
L-Alanine 89.0 18.0 0.20224719
L-Arginine hydrochloride 211.0 422.0 2.0
L-Asparagine-H2O 150.0 30.0 0.2
L-Aspartic acid 133.0 26.6 0.2
L-Cysteine hydrochloride-H2O 176.0 70.0 0.39772728
L-Glutamic Acid 147.0 29.0 0.19727892
L-Glutamine 146.0 292.0 2.0
L-Histidine hydrochloride-H2O 210.0 45.8 0.21809523
L-Isoleucine 131.0 7.88 0.060152672
L-Leucine 131.0 26.2 0.2
L-Lysine hydrochloride 183.0 73.0 0.3989071
L-Methionine 149.0 8.96 0.06013423
L-Phenylalanine 165.0 9.92 0.060121212
L-Proline 115.0 69.0 0.6
L-Serine 105.0 21.0 0.2
L-Threonine 119.0 23.0 0.19327731
L-Tryptophan 204.0 4.1 0.020098038
L-Tyrosine disodium salt dihydrate 262.0 13.5 0.051526718
L-Valine 117.0 23.0 0.1965812
Vitamins
Biotin 244.0 0.07 2.8688525E-4
Choline chloride 140.0 14.0 0.1
D-Calcium pantothenate 477.0 0.5 0.001048218
Folic Acid 441.0 1.3 0.0029478457
Niacinamide 122.0 0.037 3.032787E-4
Pyridoxine hydrochloride 206.0 0.06 2.9126214E-4
Riboflavin 376.0 0.04 1.0638298E-4
Thiamine hydrochloride 337.0 0.3 8.9020777E-4
Vitamin B12 1355.0 1.4 0.0010332103
i-Inositol 180.0 18.0 0.1
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 102.0 0.9189189
Cupric sulfate (CuSO4-5H2O) 250.0 0.0020 8.0E-6
Ferric sulfate (FeSO4-7H2O) 278.0 0.8 0.0028776978
Magnesium Chloride (anhydrous) 95.0 49.7 0.5231579
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 192.0 1.6
Potassium Chloride (KCl) 75.0 285.0 3.8
Sodium Bicarbonate (NaHCO3) 84.0 2500.0 29.761906
Sodium Chloride (NaCl) 58.0 7530.0 129.82759
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142.0 115.5 0.8133803
Sodium Phosphate monobasic (NaH2PO4) anhydrous 139.0 59.0 0.42446044
Zinc sulfate (ZnSO4-7H2O) 288.0 0.144 4.9999997E-4
Other Components
D-Glucose (Dextrose) 180.0 1260.0 7.0
Hypoxanthine Na 131.0 4.0 0.030534351
Lipoic Acid 206.0 0.21 0.0010194174
Phenol Red 376.4 3.0 0.007970245
Putrescine 2HCl 161.0 0.32 0.0019875776
Sodium Pyruvate 110.0 220.0 2.0
Thymidine 242.0 0.7 0.002892562

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