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11605 - Grace's Insect Medium, Supplemented

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 650.0 8.666667
L-Alanine 89.0 225.0 2.52809
L-Arginine hydrochloride 211.0 700.0 3.3175356
L-Asparagine 132.0 350.0 2.6515152
L-Aspartic acid 133.0 350.0 2.631579
L-Cystine 2HCl 313.0 28.68 0.09162939
L-Glutamic Acid 147.0 600.0 4.0816326
L-Glutamine 146.0 600.0 4.109589
L-Histidine 155.0 2500.0 16.129032
L-Isoleucine 131.0 50.0 0.3816794
L-Leucine 131.0 75.0 0.57251906
L-Lysine hydrochloride 183.0 625.0 3.4153006
L-Methionine 149.0 50.0 0.33557048
L-Phenylalanine 165.0 150.0 0.90909094
L-Proline 115.0 350.0 3.0434783
L-Serine 105.0 550.0 5.2380953
L-Threonine 119.0 175.0 1.4705882
L-Tryptophan 204.0 100.0 0.49019608
L-Tyrosine disodium salt 225.0 62.14 0.27617776
L-Valine 117.0 100.0 0.85470086
beta-Alanine 89.0 200.0 2.247191
Vitamins
Biotin 244.0 0.01 4.0983607E-5
Choline chloride 140.0 0.2 0.0014285714
D-Calcium pantothenate 477.0 0.02 4.192872E-5
Folic Acid 441.0 0.02 4.5351473E-5
Nicotinic acid (Niacin) 123.0 0.02 1.6260162E-4
Para-Aminobenzoic Acid 137.0 0.02 1.459854E-4
Pyridoxine hydrochloride 206.0 0.02 9.708737E-5
Riboflavin 376.0 0.02 5.319149E-5
Thiamine hydrochloride 337.0 0.02 5.934718E-5
i-Inositol 180.0 0.02 1.11111105E-4
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 500.0 4.5045047
Magnesium Chloride (anhydrous) 95.0 1070.0 11.263158
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 1358.0 11.316667
Potassium Chloride (KCl) 75.0 2800.0 37.333332
Sodium Bicarbonate (NaHCO3) 84.0 350.0 4.1666665
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 1013.0 7.3405795
Other Components
Alpha-Ketoglutaric acid 146.0 370.0 2.5342467
D-Fructose 180.0 400.0 2.2222223
D-Glucose (Dextrose) 180.0 700.0 3.8888888
Fumaric acid 116.0 55.0 0.47413793
Lactalbumin hydrolysate 3303.0 Infinity
Malic acid 134.0 670.0 5.0
Succinic acid 118.0 60.0 0.5084746
Sucrose 342.0 26680.0 78.011696
Yeastolate 3330.0 Infinity

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