Technical Resources

11530 - CMRL, no Glutamine

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 50.0 0.6666667
Hydroxy L-proline 131.0 10.0 0.07633588
L-Alanine 89.0 25.0 0.28089887
L-Arginine hydrochloride 211.0 70.0 0.33175355
L-Aspartic acid 133.0 30.0 0.22556391
L-Cysteine hydrochloride-H2O 176.0 260.0 1.4772727
L-Cystine 240.0 26.0 0.108333334
L-Glutamic Acid 147.0 75.0 0.5102041
L-Histidine hydrochloride-H2O 210.0 20.0 0.0952381
L-Isoleucine 131.0 20.0 0.15267175
L-Leucine 131.0 60.0 0.45801526
L-Lysine hydrochloride 183.0 70.0 0.38251367
L-Methionine 149.0 15.0 0.10067114
L-Phenylalanine 165.0 25.0 0.15151516
L-Proline 115.0 40.0 0.3478261
L-Serine 105.0 25.0 0.23809524
L-Threonine 119.0 30.0 0.25210086
L-Tryptophan 204.0 10.0 0.04901961
L-Tyrosine disodium salt dihydrate 262.0 58.0 0.22137405
L-Valine 117.0 25.0 0.21367522
Vitamins
Ascorbic Acid 176.0 50.0 0.2840909
Biotin 244.0 0.01 4.0983607E-5
Cholesterol 387.0 0.2 5.1679584E-4
Choline chloride 140.0 0.5 0.0035714286
D-Calcium pantothenate 477.0 0.01 2.096436E-5
Folic Acid 441.0 0.01 2.2675737E-5
Niacinamide 122.0 0.025 2.0491803E-4
Nicotinic acid (Niacin) 123.0 0.025 2.0325204E-4
Para-Aminobenzoic Acid 137.0 0.05 3.6496352E-4
Pyridoxal hydrochloride 204.0 0.025 1.2254903E-4
Pyridoxine hydrochloride 206.0 0.025 1.21359226E-4
Riboflavin 376.0 0.01 2.6595744E-5
Thiamine hydrochloride 337.0 0.01 2.967359E-5
i-Inositol 180.0 0.05 2.7777778E-4
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 200.0 1.8018018
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 97.7 0.81416667
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2200.0 26.190475
Sodium Chloride (NaCl) 58.0 6365.0 109.74138
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 140.0 1.0144928
Other Components
2'Deoxyadenosine 251.0 10.0 0.03984064
2'Deoxycytidine 227.0 10.0 0.04405286
2'Deoxyguanosine 267.0 10.0 0.037453182
5-Methyl-deoxycytidine 225.0 0.1 4.4444445E-4
Co-carboxylase 461.0 1.0 0.0021691974
Coenzyme A 768.0 2.5 0.0032552083
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
Diphosphopyridine nucleotide (NAD) 663.0 7.0 0.01055807
FAD (flavin adenine dinucleotide) 786.0 1.0 0.0012722646
Glutathione (reduced) 307.0 10.0 0.03257329
Phenol Red 376.4 20.0 0.053134963
Sodium acetate-3H2O 136.0 83.0 0.6102941
Sodium glucuronate-H2O 236.0 4.2 0.01779661
Thymidine 242.0 10.0 0.041322313
Triphosphopyridine Nucleotide (NADP) 743.0 1.0 0.001345895
Tween 80® 5.0 Infinity
Uridine 5'- triphosphate 484.0 1.0 0.0020661156

Reference:

1. Parker R.C., et al. (1957) Special Publications, N.Y. Academy of Sciences, 5, 303.

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