Technical Resources

12200 - IMDM, powder

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 30.0 0.4
L-Alanine 89.0 25.0 0.28089887
L-Arginine hydrochloride 211.0 84.0 0.39810428
L-Asparagine (freebase) 150.0 25.0 0.16666667
L-Aspartic acid 133.0 30.0 0.22556391
L-Cystine 2HCl 240.0 91.4 0.38083333
L-Glutamic Acid 147.0 75.0 0.5102041
L-Glutamine 146.0 584.0 4.0
L-Histidine hydrochloride-H2O 210.0 42.0 0.2
L-Isoleucine 131.0 105.0 0.8015267
L-Leucine 131.0 105.0 0.8015267
L-Lysine hydrochloride 183.0 146.0 0.7978142
L-Methionine 149.0 30.0 0.20134228
L-Phenylalanine 165.0 66.0 0.4
L-Proline 115.0 40.0 0.3478261
L-Serine 105.0 42.0 0.4
L-Threonine 119.0 95.0 0.79831934
L-Tryptophan 204.0 16.0 0.078431375
L-Tyrosine disodium salt 225.0 104.0 0.46222222
L-Valine 117.0 94.0 0.8034188
Vitamins
Biotin 244.0 0.013 5.327869E-5
Choline chloride 140.0 4.0 0.028571429
D-Calcium pantothenate 477.0 4.0 0.008385744
Folic Acid 441.0 4.0 0.009070295
Niacinamide 122.0 4.0 0.032786883
Pyridoxal hydrochloride 204.0 4.0 0.019607844
Riboflavin 376.0 0.4 0.0010638298
Thiamine hydrochloride 337.0 4.0 0.011869436
Vitamin B12 1355.0 0.013 9.594096E-6
i-Inositol 180.0 7.2 0.04
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 165.0 1.4864864
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 97.67 0.8139166
Potassium Chloride (KCl) 75.0 330.0 4.4
Potassium Nitrate (KNO3) 101.0 0.076 7.524752E-4
Sodium Chloride (NaCl) 58.0 4500.0 77.586205
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 125.0 0.9057971
Sodium Selenite (Na2SeO3-5H20) 263.0 0.0173 6.5779466E-5
Other Components
D-Glucose (Dextrose) 180.0 4500.0 25.0
HEPES 238.0 5958.0 25.033613
Phenol Red 376.4 15.0 0.039851222
Sodium Pyruvate 110.0 110.0 1.0

Reference:

1. Dulbecco, R. and Freeman, G. (1959) Virology 8:396. 

2. Iscove, N.N. and Melchers, F. (1978) J. Exper. Med., 147:923.

3. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) Virology 12:185.

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