Technical Resources

16600 - McCoy's 5A (modified)

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 7.5 0.1
L-Alanine 89.0 13.9 0.15617977
L-Arginine hydrochloride 211.0 42.1 0.19952606
L-Asparagine 132.0 45.0 0.3409091
L-Aspartic acid 133.0 19.97 0.15015037
L-Cysteine 121.0 31.5 0.2603306
L-Glutamic Acid 147.0 22.1 0.15034014
L-Glutamine 146.0 219.2 1.5013698
L-Histidine hydrochloride-H2O 210.0 20.96 0.09980952
L-Hydroxyproline 131.0 19.7 0.15038168
L-Isoleucine 131.0 39.36 0.300458
L-Leucine 131.0 39.36 0.300458
L-Lysine hydrochloride 183.0 36.5 0.19945355
L-Methionine 149.0 14.9 0.099999994
L-Phenylalanine 165.0 16.5 0.1
L-Proline 115.0 17.3 0.15043478
L-Serine 105.0 26.3 0.25047618
L-Threonine 119.0 17.9 0.15042016
L-Tryptophan 204.0 3.1 0.0151960775
L-Tyrosine 181.0 18.1 0.1
L-Valine 117.0 17.6 0.15042736
Vitamins
Ascorbic Acid 176.0 0.5 0.0028409092
Biotin 244.0 0.2 8.1967213E-4
Choline chloride 140.0 5.0 0.035714287
D-Calcium pantothenate 477.0 0.2 4.1928721E-4
Folic Acid 441.0 10.0 0.022675738
Niacinamide 122.0 0.5 0.0040983604
Nicotinic acid (Niacin) 123.0 0.5 0.0040650405
Para-Aminobenzoic Acid 137.0 1.0 0.00729927
Pyridoxal hydrochloride 204.0 0.5 0.0024509805
Pyridoxine hydrochloride 206.0 0.5 0.0024271845
Riboflavin 376.0 0.2 5.319149E-4
Thiamine hydrochloride 337.0 0.2 5.934718E-4
Vitamin B12 1355.0 2.0 0.0014760147
i-Inositol 180.0 36.0 0.2
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 100.0 0.9009009
Magnesium Sulfate (MgSO4-7H2O) 246.0 200.0 0.8130081
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2200.0 26.190475
Sodium Chloride (NaCl) 58.0 6460.0 111.37931
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 580.0 4.2028985
Other Components
Bacto-Peptone 600.0 Infinity
D-Glucose (Dextrose) 180.0 3000.0 16.666666
Glutathione (reduced) 307.0 0.5 0.0016286644
Phenol Red 376.4 10.0 0.026567481

Reference:

1. Hsu, T.C. and Kellogg, D.S., Jr. (1960) J. Nat. Cancer Inst., 25:221.

2. Iwakata, S. and Grace, J.T., Jr. (1964) N.Y.J. Med., 64:2279.

3. McCoy, T.A., Maxwell, M. and Kruse, P.F. (1959) Proc. Soc. Exper. Biol. Med., 100:115. 

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