Technical Resources

MCDB 131, no Glutamine

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 2.3 0.030666666
L-Alanine 89.0 2.7 0.030337078
L-Arginine hydrochloride 211.0 63.2 0.29952607
L-Asparagine-H2O 150.0 15.0 0.1
L-Aspartic acid 133.0 13.3 0.1
L-Cysteine 2HCl. H2O 176.0 35.0 0.19886364
L-Glutamic Acid 147.0 4.4 0.029931974
L-Histidine hydrochloride-H2O 210.0 42.0 0.2
L-Isoleucine 131.0 66.0 0.5038168
L-Leucine 131.0 131.0 1.0
L-Lysine hydrochloride 183.0 182.0 0.9945355
L-Methionine 149.0 15.0 0.10067114
L-Phenylalanine 165.0 33.0 0.2
L-Proline 115.0 11.5 0.1
L-Serine 105.0 32.0 0.30476192
L-Threonine 119.0 12.0 0.10084034
L-Tryptophan 204.0 4.1 0.020098038
L-Tyrosine 181.0 18.1 0.1
L-Valine 117.0 117.0 1.0
Vitamins
Biotin 244.0 0.0073 2.9918034E-5
Choline chloride 140.0 14.0 0.1
D-Calcium pantothenate 477.0 12.0 0.025157232
Folinic Acid Calcium salt 512.0 0.6 0.001171875
Niacinamide 122.0 6.1 0.05
Pyridoxine hydrochloride 206.0 2.1 0.010194174
Riboflavin 376.0 0.0038 1.0106383E-5
Thiamine hydrochloride 337.0 3.4 0.010089021
Vitamin B12 1355.0 0.0136 1.0036901E-5
i-Inositol 180.0 7.2 0.04
Inorganic Salts
Ammonium Molybdate ((NH4)6Mo7O24-4H2O) 1235.0 0.0037 2.9959515E-6
Ammonium metavanadate (NH4VO3) 121.93 6.0E-4 4.9208566E-6
Calcium Chloride (CaCl2-2H2O) 147.0 235.0 1.5986395
Cupric sulfate (CuSO4-5H2O) 250.0 0.0012 4.8E-6
Ferric sulfate (FeSO4-7H2O) 278.0 0.283 0.0010179856
Magnesium Sulfate (MgSO4-7H2O) 246.0 2464.0 10.01626
Manganese Sulfate (MnSO4-H20) 169.0 2.0E-4 1.1834319E-6
Nickelous Chloride NiCl2 6H2O 238.0 7.1E-5 2.9831935E-7
Potassium Chloride (KCl) 75.0 298.0 3.9733334
Selenious Acid H2SeO3 129.0 0.0038 2.9457366E-5
Sodium Bicarbonate (NaHCO3) 84.0 1176.0 14.0
Sodium Chloride (NaCl) 58.0 6430.0 110.86207
Sodium Meta Silicate Na2SiO3 9H2O 284.0 2.8 0.009859155
Sodium Phosphate dibasic (Na2HPO4-7H2O) 268.0 134.0 0.5
Zinc Sulfate (ZnSO4-H2O) 288.0 3.0E-4 1.0416667E-6
Other Components
Adenine 135.0 0.135 0.0010
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
Lipoic Acid 206.0 0.0021 1.0194174E-5
Phenol Red 376.4 12.4 0.032943677
Putrescine 2HCl 161.0 2.0E-4 1.242236E-6
Sodium Pyruvate 110.0 110.0 1.0
Thymidine 242.0 0.024 9.917356E-5

Reference:

1. Knedler and Ham (1987) In Vitro Cellular and Developmental Biology, 23, 481.

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