Technical Resources

11825 - Medium 199, 10x

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 500.0 6.6666665
L-Alanine 89.0 250.0 2.8089888
L-Arginine hydrochloride 211.0 700.0 3.3175356
L-Aspartic acid 133.0 300.0 2.255639
L-Cysteine hydrochloride-H2O 176.0 1.1 0.00625
L-Cystine 2HCl 313.0 260.0 0.83067095
L-Glutamic Acid-H2O 165.0 750.0 4.5454545
L-Histidine hydrochloride-H2O 210.0 218.8 1.0419048
L-Hydroxyproline 131.0 100.0 0.7633588
L-Isoleucine 131.0 400.0 3.053435
L-Leucine 131.0 600.0 4.5801525
L-Lysine hydrochloride 183.0 700.0 3.8251367
L-Methionine 149.0 150.0 1.0067114
L-Phenylalanine 165.0 250.0 1.5151515
L-Proline 115.0 400.0 3.4782608
L-Serine 105.0 250.0 2.3809524
L-Threonine 119.0 300.0 2.5210085
L-Tryptophan 204.0 100.0 0.49019608
L-Tyrosine disodium salt dihydrate 261.0 580.0 2.2222223
L-Valine 117.0 250.0 2.1367521
Vitamins
Ascorbic Acid 176.0 0.5 0.0028409092
Biotin 244.0 0.1 4.0983607E-4
Choline chloride 140.0 5.0 0.035714287
D-Calcium pantothenate 477.0 0.1 2.0964361E-4
Folic Acid 441.0 0.1 2.2675737E-4
Menadione (Vitamin K3) 172.0 0.1 5.8139535E-4
Niacinamide 122.0 0.25 0.0020491802
Nicotinic acid (Niacin) 123.0 0.25 0.0020325202
Para-Aminobenzoic Acid 137.0 0.5 0.003649635
Pyridoxal hydrochloride 204.0 0.25 0.0012254902
Pyridoxine hydrochloride 206.0 0.25 0.0012135922
Riboflavin 376.0 0.1 2.6595744E-4
Thiamine hydrochloride 337.0 0.1 2.967359E-4
Vitamin A (acetate) 328.0 1.0 0.0030487804
Vitamin D2 (Calciferol) 397.0 1.0 0.0025188916
alpha Tocopherol phos. Na salt 554.7 0.1 1.8027762E-4
i-Inositol 180.0 0.5 0.0027777778
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 2000.0 18.018019
Ferric nitrate (Fe(NO3)-9H2O) 404.0 7.2 0.017821781
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 970.0 8.083333
Potassium Chloride (KCl) 75.0 4000.0 53.333332
Sodium Chloride (NaCl) 58.0 68000.0 1172.4138
Sodium Phosphate monobasic (NaH2PO4) anhydrous 138.0 1400.0 10.144928
Other Components
2-deoxy-D-ribose 134.0 5.0 0.03731343
Adenine sulfate 404.0 100.0 0.24752475
Adenosine 5'-phosphate 347.0 2.0 0.0057636886
Adenosine 5'-triphosphate 605.0 10.0 0.016528925
Cholesterol 387.0 2.0 0.005167959
D-Glucose (Dextrose) 180.0 10000.0 55.555557
Glutathione (reduced) 307.0 0.5 0.0016286644
Guanine hydrochloride 188.0 3.0 0.015957447
Hypoxanthine Na 158.0 3.5 0.022151899
Phenol Red 376.4 200.0 0.53134966
Ribose 150.0 5.0 0.033333335
Sodium Acetate 82.0 500.0 6.097561
Thymine 126.0 3.0 0.023809524
Tween 80® 200.0 Infinity
Uracil 112.0 3.0 0.026785715
Xanthine-Na 152.0 3.44 0.02263158

Reference:

1. Morgan, J.F. and Campbell, M.E. (1955) J. Natl. Cancer Inst., 16:557.

2. Morgan, J.F., Morton, H.J. and Parker R.C. (1950) Proc. Soc. Exp. Biol. Med., 73:1.

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