Technical Resources

12000 - MEM alpha, no nucleosides, powder

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 50.0 0.6666667
L-Alanine 89.0 25.0 0.28089887
L-Arginine 211.0 105.0 0.49763033
L-Asparagine-H2O 132.0 50.0 0.37878788
L-Aspartic acid 133.0 30.0 0.22556391
L-Cysteine hydrochloride-H2O 176.0 100.0 0.5681818
L-Cystine 2HCl 240.0 24.0 0.1
L-Glutamic Acid 147.0 75.0 0.5102041
L-Glutamine 146.0 292.0 2.0
L-Histidine 155.0 31.0 0.2
L-Isoleucine 131.0 52.4 0.4
L-Leucine 131.0 52.4 0.4
L-Lysine 146.0 58.0 0.39726028
L-Methionine 149.0 15.0 0.10067114
L-Phenylalanine 165.0 32.0 0.19393939
L-Proline 115.0 40.0 0.3478261
L-Serine 105.0 25.0 0.23809524
L-Threonine 119.0 48.0 0.40336135
L-Tryptophan 204.0 10.0 0.04901961
L-Tyrosine disodium salt 225.0 52.0 0.23111111
L-Valine 117.0 46.0 0.3931624
Vitamins
Ascorbic Acid 176.0 50.0 0.2840909
Biotin 244.0 0.1 4.0983607E-4
Choline chloride 140.0 1.0 0.007142857
D-Calcium pantothenate 477.0 1.0 0.002096436
Folic Acid 441.0 1.0 0.0022675737
Niacinamide 122.0 1.0 0.008196721
Pyridoxal hydrochloride 204.0 1.0 0.004901961
Riboflavin 376.0 0.1 2.6595744E-4
Thiamine hydrochloride 337.0 1.0 0.002967359
Vitamin B12 1355.0 1.36 0.0010036901
i-Inositol 180.0 2.0 0.011111111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 200.0 1.8018018
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 97.67 0.8139166
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Chloride (NaCl) 58.0 6800.0 117.24138
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 140.0 1.0144928
Other Components
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
Lipoic Acid 206.0 0.2 9.708738E-4
Phenol Red 376.4 10.0 0.026567481
Sodium Pyruvate 110.0 110.0 1.0

Reference:

1. Simonsen, C.C. and Levinson, A.D. 1983. Isolation and expression of an altered mouse dihydrofolate reductase cDNA. Proc. Natl. Acad. Sci. U.S.A. 80:2495-2499.

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