Proliferating cells labeled in vivo with the Click-iT® EdU assay.
EdU was administered to mice intraperitoneally 2 hours before sacrifice. After intestinal tissues were formalin fixed and paraffin embedded, EdU was labeled with the Click-iT® reaction from the Click-iT® EdU Alexa Fluor® 488 Imaging Assay (Cat. no. C10337), using approximately 250 µL of reaction cocktail per slide. The tissue sections were then washed and treated with mounting media containing DAPI, EdU-positive cells are labeled green, nuclei are stained blue and red autofluorescence was enhanced for image contrast.
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
Cell proliferation detected with Click-iT® EdU and counter stained with anti-LC3B and a goat anti-rabbit Alexa Fluor® 647 secondary antibody. Go ›
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›
Muntjac fibroblast labeled with probes for actin and the nucleus. Go ›
Proliferating cells in rat ileum using Click-iT® EdU. Go ›
Using anti-LC3B and the Click-iT® technology autophagy detected and glycoproteins labeled in HeLa cells Go ›