Immunocytochemistry image of A21975, peroxisomal multifunctional enzyme 2 (MFE2) monoclonal antibody.
Human HDFn cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody at 5 µg/mL overnight at 4°C. Secondary antibody: (red) Alexa Fluor® 594 Goat Anti-Mouse IgG (H+L) at a 1/1000 dilution for 1 hr. 10% goat serum was used as the blocking agent for all blocking steps. Target protein locates mainly in peroxisome.
Madin-Darby canine kidney cells. LysoSensor Yellow/Blue DND-160. Go ›
Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran. Go ›
Enhanced chromogenic substrate detection using Thermo Scientific Pierce Western Blot Signal Enhancer Go ›
Enhanced chemiluminescent substrate detection using Thermo Scientific Pierce Western Blot Signal Enhancer Go ›
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›
Muntjac fibroblast labeled with probes for actin and the nucleus. Go ›