Neonatal human dermal fibroblasts fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit.

Human dermal fibroblasts, neonatal (HDFn) (C-004-5C) were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602). Golgi staining was done using an anti-Golgin-97 primary antibody (A21270) and a goat anti-mouse Alexa Fluor® 488 secondary antibody (A11029). Actin was stained using Alexa Fluor® 594 phalloidin (A12381) and nuclei were stained using NucBlue™ Live Cell Stain (R37605). Slides were mounted using ProLong® Gold antifade kit (P7481) and images were acquired on the FLoid™ Cell Imaging Station (4471136).

Human dermal fibroblasts, neonatal (HDFn) (C-004-5C) were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602). Golgi staining was done using an anti-Golgin-97 primary antibody (A21270) and a goat anti-mouse Alexa Fluor® 488 secondary antibody (A11029). Actin was stained using Alexa Fluor® 594 phalloidin (A12381) and nuclei were stained using NucBlue™ Live Cell Stain (R37605). Slides were mounted using ProLong® Gold antifade kit (P7481) and images were acquired on the FLoid™ Cell Imaging Station (4471136).

Related Products

Sku Name Size Price Qty
4471136 FLoid® Cell Imaging Station 1 each USD 13,300.00
A11029 Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L) Antibody, highly cross-adsorbed 0.5 mL USD 199.00
A12381 Alexa Fluor® 594 Phalloidin 300 units USD 373.00
A21270 Golgin-97 Monoclonal Antibody, Mouse (CDF4) 100 µg USD 220.00
C0045C Human Dermal Fibroblasts, neonatal (HDFn) 1 vial USD 371.00
R37602 Image-iT® Fixation⁄Permeabilization Kit 1 kit USD 250.00
R37605 NucBlue® Live ReadyProbes® Reagent 1 kit USD 97.00

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