Cell proliferation detected with Click-iT® EdU and counter stained with anti-LC3B and a goat anti-rabbit Alexa Fluor® 647 secondary antibody.
HeLa cells were treated with 30 µM chloroquine and cultured overnight. The following day, the cells were fed 10 µM EdU under regular growth conditions for one hour and then fixed and permeabilized. EdU was used to was visualize proliferating cells using The Click-iT® EdU Alexa Fluor® 488 Imaging kit (pink). Cells were counter stained with 0.5 µg/mL anti-LC3B with a goat anti rabbit Alexa Fluor® 647 secondary (Green), mouse anti alpha tubulin with a goat anti mouse Alexa Fluor® 555 secondary (Cyan) and 1 µg/mL Hoechst 33342 (Blue). Cells were imaged on a Molecular Devices ImageXpress High content imager.
Three-color staining of HeLa cells using fluorescent Qdot® nanocrystal conjugates. Go ›
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody Go ›
Human tonsil section visualized with fluorescent Qdot® nanocrystal conjugates. Go ›
Developing Drosophila embryo Go ›
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
MagicMedia™ protocol Go ›
Higher protein yields achieved with the MagicMedia™ E. coli Expression Medium Go ›
Immunoflorecence using NF-kappa-B (p65) Monoclonal Antibody, Mouse Go ›
Human iPSC staining using mouse anti beta-tubulin monoclonal antibody. Go ›
Labeled paint probes hybridized to human metaphase chromosomes Go ›
Cytoskeleton of a mixed population of granule neurons and glial cells Go ›
Proliferating cells in rat ileum using Click-iT® EdU. Go ›