Cell proliferation detected with Click-iT® EdU and counter stained with anti-LC3B and a goat anti-rabbit Alexa Fluor® 647 secondary antibody.
HeLa cells were treated with 30 µM chloroquine and cultured overnight. The following day, the cells were fed 10 µM EdU under regular growth conditions for one hour and then fixed and permeabilized. EdU was used to was visualize proliferating cells using The Click-iT® EdU Alexa Fluor® 488 Imaging kit (pink). Cells were counter stained with 0.5 µg/mL anti-LC3B with a goat anti rabbit Alexa Fluor® 647 secondary (Green), mouse anti alpha tubulin with a goat anti mouse Alexa Fluor® 555 secondary (Cyan) and 1 µg/mL Hoechst 33342 (Blue). Cells were imaged on a Molecular Devices ImageXpress High content imager.
Muntjac fibroblast labeled with probes for actin and the nucleus. Go ›
Multicolor fluorescence analysis of muntjac fibroblasts. Go ›
Simultaneous detection of expression of five genes in a whole mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes Go ›
Nuclear, cytoskeletal, and mitochondrial staining of Muntjac fibroblasts. Go ›
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
Table 2 - Tris-Glycine/Tricine to NuPAGE® Gel conversion chart Go ›
Table 1 - Migration patterns of protein standards* on NuPAGE® Gels Go ›
Western blot analysis of (A) MDA-MB-231, (B) MDA-MB-468, (C) TF-1, (D) K562, (E) Caco-2, and (F) PC-3 cell lysates, and (G) mouse testis homogenates using our Rb anti-Metadherin (C-term) (Cat. No. 40-6400). Go ›
Immunoflorecence using NF-kappa-B (p65) Monoclonal Antibody, Mouse Go ›
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody Go ›
Labeled paint probes hybridized to human metaphase chromosomes Go ›
Proliferating cells in rat ileum using Click-iT® EdU. Go ›