Phagocytosis in MMM Cells using pHrodo™ Red E. coli BioParticles® Conjugate® (Catalog # P35361)
MMM macrophage cells were plated in complete medium and left overnight in cell culture. The following day, cells were rinsed 1X with Live Cell Imaging Solution (catalog # A14291DJ) and replaced pHrodo™ Red E. coli BioParticles® Conjugate® (Catalog # P35361) were resuspended at working concentration in Live Cell Imaging Solution at 1 mg/mL, and added to the cells, then plates moved back to 37 °C for 90 minutes incubation. NucBlue™ Live Cell Stain *Hoechst 33342 special formulation* (Catalog # R37605), was added to stain the nucleus.
Muntjac skin fibroblast labeled with rhodamine phalloidin. Go ›
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody Go ›
Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran. Go ›
Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›
Table 2 - Tris-Glycine/Tricine to NuPAGE® Gel conversion chart Go ›
Table 1 - Migration patterns of protein standards* on NuPAGE® Gels Go ›