Phagocytosis in MMM Cells using pHrodo™ Red E. coli BioParticles® Conjugate® (Catalog # P35361)
MMM macrophage cells were plated in complete medium and left overnight in cell culture. The following day, cells were rinsed 1X with Live Cell Imaging Solution (catalog # A14291DJ) and replaced pHrodo™ Red E. coli BioParticles® Conjugate® (Catalog # P35361) were resuspended at working concentration in Live Cell Imaging Solution at 1 mg/mL, and added to the cells, then plates moved back to 37 °C for 90 minutes incubation. NucBlue™ Live Cell Stain *Hoechst 33342 special formulation* (Catalog # R37605), was added to stain the nucleus.
Muntjac skin fibroblast labeled with rhodamine phalloidin. Go ›
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody Go ›
Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran. Go ›
Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›