Phagocytosis in MMM Cells using pHrodo™ Red E. coli BioParticles® Conjugate® (Catalog # P35361)

MMM macrophage cells were plated in complete medium and left overnight in cell culture. The following day, cells were rinsed 1X with Live Cell Imaging Solution (catalog # A14291DJ) and replaced pHrodo™ Red E. coli BioParticles® Conjugate® (Catalog # P35361) were resuspended at working concentration in Live Cell Imaging Solution at 1 mg/mL, and added to the cells, then plates moved back to 37 °C for 90 minutes incubation. NucBlue™ Live Cell Stain *Hoechst 33342 special formulation* (Catalog # R37605), was added to stain the nucleus.

MMM macrophage cells were plated in complete medium and left overnight in cell culture.  The following day, cells were rinsed 1X with Live Cell Imaging Solution (catalog # A14291DJ) and replaced pHrodo™ Red E. coli BioParticles® Conjugate® (Catalog # P35361) were resuspended at  working concentration in Live Cell Imaging Solution at 1 mg/mL, and added to the cells, then plates moved back to 37 °C for 90 minutes incubation. NucBlue™ Live Cell Stain *Hoechst 33342 special formulation* (Catalog # R37605), was added to stain the nucleus.

Related Products

Sku Name Size Price Qty
P35361 pHrodo™ Red E. coli BioParticles® Conjugate for Phagocytosis 5 x 2 mg USD 298.00
A14291DJ Live Cell Imaging Solution 500 mL USD 30.00
R37605 NucBlue® Live ReadyProbes® Reagent 1 kit USD 97.00

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