Human iPSC staining using mouse anti beta-tubulin monoclonal antibody.

Human iPSCs were cultured on glass slides under feeder-free conditions in StemPro® hESC Medium (A1000701). Cells were fixed and permed with the Image-iT® Fixation/Permeabilization Kit (R37602). Oct4 (green) expression was visualized using anti-Oct4 primary Ab and Alexa Fluor® 488 secondary Ab (A11034). Tubulin (red) expression was visualized using anti-tubulin primary Ab (322600) and Alexa Fluor® 594 secondary Ab (A11005). Nuclei (blue) were labeled with NucBlue™ Fixed Cell Stain (R37606). Images were collected on the FLoid™ Cell Imaging Station (4471136).

Human iPSCs were cultured on glass slides under feeder-free conditions in StemPro® hESC Medium (A1000701). Cells were fixed and permed with the Image-iT® Fixation/Permeabilization Kit (R37602). Oct4 (green) expression was visualized using anti-Oct4 primary Ab and Alexa Fluor® 488 secondary Ab (A11034). Tubulin (red) expression was visualized using anti-tubulin primary Ab (322600) and Alexa Fluor® 594 secondary Ab (A11005). Nuclei (blue) were labeled with NucBlue™ Fixed Cell Stain (R37606). Images were collected on the FLoid™ Cell Imaging Station (4471136).

Related Products

Sku Name Size Price Qty
322600 Beta-Tubulin mAb, Mouse (clone 2 28 33) 100 µg USD 299.00
4471136 FLoid® Cell Imaging Station 1 each USD 18,900.00
A1000701 StemPro® hESC SFM 1 kit USD 263.00
A11005 Alexa Fluor® 594 Goat Anti-Mouse IgG (H+L) Antibody 0.5 mL USD 149.00
R37602 Image-iT® Fixation⁄Permeabilization Kit 1 kit USD 250.00
R37606 NucBlue® Fixed Cell ReadyProbes® Reagent 1 kit USD 97.00
A11034 Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) Antibody, highly cross-adsorbed 0.5 mL USD 199.00
P36934 ProLong® Gold Antifade Reagent 5 x 2 mL USD 159.00

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