Fixed and stained HDFn cells.

HDFn cells were fixed, permeabilized, and blocked using the Image-iT® Fixation/Permeabilization Kit (R37602). Golgi were labeled with an anti-golgin 97 antibody (A21270) followed by detection with an Alexa Fluor® 488 secondary (A11029). Cells were also stained with Alexa Fluor® 594 phalloidin (A12381) to label actin and NucBlue™ Fixed (R37606) to label nuclei. Finally, cells were mounted in ProLong® Gold antifade reagent.

HDFn cells were fixed, permeabilized, and blocked using the Image-iT® Fixation/Permeabilization Kit (R37602). Golgi were labeled with an anti-golgin 97 antibody (A21270) followed by detection with an Alexa Fluor® 488 secondary (A11029). Cells were also stained with Alexa Fluor® 594 phalloidin (A12381) to label actin and NucBlue™ Fixed (R37606) to label nuclei. Finally, cells were mounted in ProLong® Gold antifade reagent.

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A11029 Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L) Antibody, highly cross-adsorbed 0.5 mL USD 199.00
A12381 Alexa Fluor® 594 Phalloidin 300 units USD 373.00
A21270 Golgin-97 Monoclonal Antibody, Mouse (CDF4) 100 µg USD 220.00
C0045C Human Dermal Fibroblasts, neonatal (HDFn) 1 vial USD 371.00
P36930 ProLong® Gold Antifade Mountant 10 mL USD 142.00
P36934 ProLong® Gold Antifade Mountant 5 x 2 mL USD 159.00
R37602 Image-iT® Fixation⁄Permeabilization Kit 1 kit USD 250.00
R37606 NucBlue® Fixed Cell ReadyProbes® Reagent 1 kit USD 97.00

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