Normalization with housekeeping genes on a single blot
Simultaneous detection of a protein of interest and a loading control on a single blot using WesternDot® secondary antibodies. A western blot containing a dilution series of recombinant AKT in a background of Jurkat lysate at a constant loading of 5 µg total protein. The blot was probed with mouse anti-AKT and rabbit anti-GAPDH, followed by WesternDot™ 655 goat anti-mouse (Cat. no. W10813, pseudocolored green) and WesternDot™ 800 goat anti-rabbit (Cat. No. W10816, pseudocolored red) conjugates and imaged using the Fujifilm® FLA-9000 imager.
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›