Normalization with housekeeping genes on a single blot

Simultaneous detection of a protein of interest and a loading control on a single blot using WesternDot® secondary antibodies. A western blot containing a dilution series of recombinant AKT in a background of Jurkat lysate at a constant loading of 5 µg total protein. The blot was probed with mouse anti-AKT and rabbit anti-GAPDH, followed by WesternDot™ 655 goat anti-mouse (Cat. no. W10813, pseudocolored green) and WesternDot™ 800 goat anti-rabbit (Cat. No. W10816, pseudocolored red) conjugates and imaged using the Fujifilm® FLA-9000 imager.

Simultaneous detection of a protein of interest and a loading control on a single blot using WesternDot® secondary antibodies. A western blot containing a dilution series of recombinant AKT in a background of Jurkat lysate at a constant loading of 5 µg total protein.  The blot was probed with mouse anti-AKT and rabbit anti-GAPDH, followed by WesternDot™ 655 goat anti-mouse (Cat. no. W10813, pseudocolored green) and WesternDot™ 800 goat anti-rabbit (Cat. No. W10816, pseudocolored red) conjugates and imaged using the Fujifilm® FLA-9000 imager.

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W10808 WesternDot® 625 Goat Anti-Mouse Secondary Antibody (VIVID® Technology) 25 blots USD 139.00
W10809 WesternDot® 625 Goat Anti-Rabbit Secondary Antibody (VIVID® Technology) 25 blots USD 139.00
W10810 WesternDot® 655 Donkey Anti-Mouse Secondary Antibody (VIVID® Technology) 25 blots USD 139.00
W10811 WesternDot® 655 Donkey Anti-Rabbit Secondary Antibody (VIVID® Technology) 25 blots USD 139.00
W10812 WesternDot® 655 Donkey Anti-Goat Secondary Antibody (VIVID® Technology) 25 blots USD 139.00
W10813 WesternDot® 655 Goat Anti-Mouse Secondary Antibody (VIVID® Technology) 25 blots USD 139.00
W10814 WesternDot® 655 Goat Anti-Rabbit Secondary Antibody (VIVID® Technology) 25 blots USD 139.00
W10815 WesternDot® 800 Goat Anti-Mouse Secondary Antibody (VIVID® Technology) 25 blots USD 139.00
W10816 WesternDot® 800 Goat Anti-Rabbit Secondary Antibody (VIVID® Technology) 25 blots USD 139.00

Related Images