BALB/c splenocytes were stimulated for 3 days with plate-bound Anti-Mouse CD3e Functional Grade Purified, soluble Anti-Mouse CD28 Functional Grade Purified, Mouse IL-2 Recombinant Protein, and Mouse IL-4 Recombinant Protein. Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) for 5 hours.

BALB/c splenocytes were stimulated for 3 days with plate-bound Anti-Mouse CD3e Functional Grade Purified, soluble Anti-Mouse CD28 Functional Grade Purified, Mouse IL-2 Recombinant Protein, and Mouse IL-4 Recombinant Protein. Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) for 5 hours. Following restimulation, cells were fixed and permeabilized then stained with Anti-Mouse CD4 FITC and 0.125 ug of Rat IgG1 K Isotype Control PE or 0.125 ug of Anti-Mouse IL-13 PE (A18492). Viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

BALB/c splenocytes were stimulated for 3 days with plate-bound Anti-Mouse CD3e Functional Grade Purified, soluble Anti-Mouse CD28 Functional Grade Purified, Mouse IL-2 Recombinant Protein, and Mouse IL-4 Recombinant Protein. Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) for 5 hours. Following restimulation, cells were fixed and permeabilized then stained with Anti-Mouse CD4 FITC and 0.125 ug of Rat IgG1 K Isotype Control PE or 0.125 ug of Anti-Mouse IL-13 PE (A18492). Viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

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