Multiplexing Click-iT® Plus OPP, GFP, and phalloidin
CellLight® Talin-GFP, BacMam 2.0 (Cat. No. C10611) was used to transduce HeLa cells. After overnight incubation, the Click-iT® OPP reagent was added and incubated for 30 minutes. The cells were then fixed and permeabilized, and treated with Alexa Fluor® 568 Phalloidin (Cat. No. A12380). The labeling of the nascent protein synthesis was completed using the Click-iT® Plus OPP Alexa Fluor® 647 Protein Synthesis Assay Kit (Cat. No. C10458).
Green—indentifies the binding of the talin-GFP fusion protein to the cytoskeleton.
Red—Alexa Fluor®-Phalloidin, identifies the F-actin staining within the cell
Blue punctuate pattern within the nucleus—nascent protein synthesis detected by application of the Click-iT® Plus OPP Alexa Fluor® 647 Protein Synthesis Assay
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›