Enrichment of the Cas9- and gRNA-expressing cell population
(A) Transfection efficiency in 293T cells using the GeneArt® CRISPR Nuclease OFP Vector encoding crRNA specific for the RelA locus. Data shows >90% OFP-positive cells in transfected samples. (B) CD4 functionality for the GeneArt® CRISPR Nuclease CD4 Vector. 293 FT cells were transfected with AAVS1-specific GeneArt® CRISPR Nuclease CD4 Vector. Cells were harvested and stained with anti-CD4 FITC antibody and analyzed by flow cytometry for measuring transfection efficiency. A portion of the stained cells were also seeded on a plate for analysis by fluorescence microscopy.