Bead-based enrichment using the GeneArt® CRISPR Nuclease Vector with CD4.

The sample had very low CD4-expressing cell populations and hence lower cleavage efficiency (1.4%). Following transfection, CD4-expressing cells were enriched from the rest of the cell population using anti-CD4 antibody–coated magnetic beads. A GeneArt® Genomic Cleavage Detection Assay (Cat. No. A24372) was performed on both pre- and post-enriched cell pellets. Cleavage efficiency increased to 48.1% after enrichment.

The sample had very low CD4-expressing cell populations and hence lower cleavage efficiency (1.4%). Following transfection, CD4-expressing cells were enriched from the rest of the cell population using anti-CD4 antibody–coated magnetic beads. A GeneArt® Genomic Cleavage Detection Assay (Cat. No. A24372) was performed on both pre- and post-enriched cell pellets. Cleavage efficiency increased to 48.1% after enrichment.

Related Products

Sku Name Size Price Qty
A21175 GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit 10 reactions USD 495.00
A21177 GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells) 10 reactions USD 625.00
A24372 GeneArt® Genomic Cleavage Detection Kit 20 reactions USD 220.00

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