Bead-based enrichment using the GeneArt® CRISPR Nuclease Vector with CD4.
The sample had very low CD4-expressing cell populations and hence lower cleavage efficiency (1.4%). Following transfection, CD4-expressing cells were enriched from the rest of the cell population using anti-CD4 antibody–coated magnetic beads. A GeneArt® Genomic Cleavage Detection Assay (Cat. No. A24372) was performed on both pre- and post-enriched cell pellets. Cleavage efficiency increased to 48.1% after enrichment.