Sandwich ELISA analysis of ABfinity™ EGF Recombinant Rabbit Monoclonal Antibody

Sandwich ELISA analysis was performed using ABfinity™ EGF Recombinant Rabbit Monoclonal Antibody at 2 µg/mL. A ten point standard curve was plotted with full length active recombinant EGF protein as a standard with concentrations ranging from 0.3 pg/mL to 12.5 ng/mL. An anti-EGF antibody conjugated to biotin was used as a detector at a concentration of 2 µg/mL. A non-linear regression analysis (4 PL) was performed and LOD and LOQ for the antibody pair was determined.

Sandwich ELISA analysis was performed using ABfinity™ EGF Recombinant Rabbit Monoclonal Antibody at 2 µg/mL. A ten point standard curve was plotted with full length active recombinant EGF protein as a standard with concentrations ranging from 0.3 pg/mL to 12.5 ng/mL. An anti-EGF antibody conjugated to biotin was used as a detector at a concentration of 2 µg/mL. A non-linear regression analysis (4 PL) was performed and LOD and LOQ for the antibody pair was determined.

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Sku Name Size Price Qty
701538 EGF Monoclonal Antibody, ABfinity™ Recombinant 100 µg USD 308.00

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