Maize leaf section labeled with Alexa Fluor® 488 Goat Anti–Rabbit IgG.

A 2.0 µm maize leaf section illustrating the immunolocalization of the enzyme ribulose bisphosphate carboxylase (rubisco) in the chloroplasts of the bundle sheath cells surrounding the vascular bundles. Maize is a C4 plant and, as a result, spatially segregates components of the photosynthetic process between the leaf mesophyll and the bundle sheath. Rubisco was localized using a rabbit anti-rubisco antibody and visualized using the highly cross-adsorbed Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11034). The remaining fluorescence is due to the autofluorescence of chlorophyll, which appears red and is localized to the mesophyll plastids; lignin, which appears dull green and is localized to the xylem of the vascular bundle; and cutin, which appears bright green and is localized to the cuticle outside the epidermis. Image contributed by Todd Jones, DuPont.

A 2.0 µm maize leaf section illustrating the immunolocalization of the enzyme ribulose bisphosphate carboxylase (rubisco) in the chloroplasts of the bundle sheath cells surrounding the vascular bundles. Maize is a C4 plant and, as a result, spatially segregates components of the photosynthetic process between the leaf mesophyll and the bundle sheath. Rubisco was localized using a rabbit anti-rubisco antibody and visualized using the highly cross-adsorbed Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11034). The remaining fluorescence is due to the autofluorescence of chlorophyll, which appears red and is localized to the mesophyll plastids; lignin, which appears dull green and is localized to the xylem of the vascular bundle; and cutin, which appears bright green and is localized to the cuticle outside the epidermis.  Image contributed by Todd Jones, DuPont.

Related Products

Sku Name Size Price Qty
A11034 Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) Antibody, highly cross-adsorbed 0.5 mL USD 199.00
I37156 Image-iT® FX Kit #7 (with Alexa Fluor® 488 Goat Anti-Rabbit IgG, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00

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