Maize leaf section labeled with Alexa Fluor® 488 Goat Anti–Rabbit IgG.
A 2.0 µm maize leaf section illustrating the immunolocalization of the enzyme ribulose bisphosphate carboxylase (rubisco) in the chloroplasts of the bundle sheath cells surrounding the vascular bundles. Maize is a C4 plant and, as a result, spatially segregates components of the photosynthetic process between the leaf mesophyll and the bundle sheath. Rubisco was localized using a rabbit anti-rubisco antibody and visualized using the highly cross-adsorbed Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11034). The remaining fluorescence is due to the autofluorescence of chlorophyll, which appears red and is localized to the mesophyll plastids; lignin, which appears dull green and is localized to the xylem of the vascular bundle; and cutin, which appears bright green and is localized to the cuticle outside the epidermis. Image contributed by Todd Jones, DuPont.