Immunofluorescence analysis of Mouse V5 tag - FITC Mouse Monoclonal Antibody
Immunofluorescence analysis of V5 tag was done on HEK-293 cells transiently overexpressing V5-His-LacZ. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with V5 tag-FITC Mouse Monoclonal Antibody (R96325) conjugated with FITC at a dilution of 1:400 in 1% BSA and incubated for 3 hours at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin (A12381). Panel d is a merged image showing cellular localization. Panel e is untransfected HEK-293 cells. The images were captured using a Nikon microscope at 20X magnification.