Western Blot analysis of Mouse V5 tag - FITC Mouse Monoclonal Antibody

Western blot analysis of V5 tag was performed by loading 20 µg of whole cell extracts of untransfected HEK-293 and HEK-293 transiently overexpressing V5-His- LacZ using Novex® NuPAGE® 4-12% Bis-Tris gel (NP0321BOX), XCell SureLock™ Electrophoresis System (EI0002), and Novex® Sharp Pre-Stained Protein Standard (LC5800). Proteins were transferred to a PVDF membrane and blocked with 5% skim milk for 1 hour at room temperature. V5-His- LacZ was detected at ~117 kDa using Mouse V5 tag - FITC Mouse Monoclonal Antibody (R96325) at 1:3000 dilution in 2.5% skim milk at 4°C overnight on a rocking platform. Detection was performed and image captured at an FITC emission wavelength of 518nm.

Western blot analysis of V5 tag was performed by loading 20 µg of whole cell extracts of untransfected HEK-293 and HEK-293 transiently overexpressing V5-His- LacZ using Novex® NuPAGE® 4-12% Bis-Tris gel (NP0321BOX), XCell SureLock™ Electrophoresis System (EI0002), and Novex® Sharp Pre-Stained Protein Standard (LC5800). Proteins were transferred to a PVDF membrane and blocked with 5% skim milk for 1 hour at room temperature. V5-His- LacZ was detected at ~117 kDa using Mouse V5 tag - FITC Mouse Monoclonal Antibody (R96325) at 1:3000 dilution in 2.5% skim milk at 4°C overnight on a rocking platform. Detection was performed and image captured at an FITC emission wavelength of 518nm.

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Catalog # Name Size List Price (USD) Qty
R96325 V5 Tag Monoclonal Antibody, Mouse FITC Conjugate 50 µL 260.00

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