Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody

Golgi and actin staining in HeLa cells. Golgi in fixed and permeabilized HeLa cells labeled with anti–golgin-97 monoclonal antibody (Cat. No. A21270) and visualized with green-fluorescent Alexa Fluor® 488 Goat-Anti–Mouse IgG (Cat. No. A11001). Actin was stained with red-fluorescent Alexa Fluor® 594 phalloidin (Cat. No. A12381); nuclei were stained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490). Treatment with Image-iT® FX signal enhancer (Cat. No. I36933) largely eliminates nonspecific dye binding (bottom) as compared to untreated slide (top).

Golgi and actin staining in HeLa cells. Golgi in fixed and permeabilized HeLa cells labeled with anti–golgin-97 monoclonal antibody (Cat. No. A21270) and visualized with green-fluorescent Alexa Fluor® 488 Goat-Anti–Mouse IgG (Cat. No. A11001). Actin was stained with red-fluorescent Alexa Fluor® 594 phalloidin (Cat. No. A12381); nuclei were stained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490). Treatment with Image-iT® FX signal enhancer (Cat. No. I36933) largely eliminates nonspecific dye binding (bottom) as compared to untreated slide (top).

Related Products

Sku Name Size Price Qty
A12381 Alexa Fluor® 594 Phalloidin 300 units USD 373.00
D1306 DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) 10 mg USD 89.00
D21490 DAPI, FluoroPure™ grade 10 mg USD 133.00
D3571 DAPI (4',6-Diamidino-2-Phenylindole, Dilactate) 10 mg USD 89.00
I36933 Image-iT® FX Signal Enhancer 10 mL USD 98.75
A11001 Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L) Antibody 0.5 mL USD 149.00
A21270 Golgin-97 Monoclonal Antibody, Mouse (CDF4) 100 µg USD 220.00

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