Adult rat cortical astrocyte. Rhod-2 AM and MitoFluor™ Green stain.
Colocalization of fluorescent staining by rhod-2 AM (Cat. no. R1244, R1245MP; upper panel) and mitochondrion-selective MitoFluor™ Green stain (Cat. no. M7502; lower panel) in an adult rat cortical astrocyte. Cells were simultaneously loaded with rhod-2 AM (4.5 µM) and the MitoFluor™ Green stain (20 nM) for 30 minutes at 22°C. Confocal laser-scanning microscopy using 488 nm excitation and spectrally resolved detection, at 505–530 nm for MitoFluor™ Green stain and >=585 nm for rhod-2, shows almost identical staining distribution. The images were contributed by Michael Duchen, University College, London. Reproduced with permission from J Cell Biol 145, 795 (1999).
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
Pseudocolored images of changes in intracellular free Ca2+ in AtT-20/D16v-F2 cells, monitored at 9 sec intervals with fluo-4, AM (F14201, F14202, F14217, F23917). Go ›
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›
Multicolor fluorescence analysis of muntjac fibroblasts. Go ›