Amplification of FISH signals using the Alexa Fluor® 488 Signal-Amplification Kit for Fluorescein- and Oregon Green® Dye–Conjugated Probes.

Chromosome spreads were prepared from the cultured fibroblast cell line MRC-5 and hybridized with an a-satellite probe labeled with the Oregon Green® 488 dye and specific for chromosome 17. The probe was labeled using the ULYSIS Alexa Fluor® 488 Nucleic Acid Labeling Kit (Cat. No. U21659) (left panel). The signal was amplified using the Alexa Fluor® 488 conjugate of rabbit anti-fluorescein/Oregon Green® antibody (Cat. No. A11090; also available in Cat. No. A11053) (middle panel) and amplified once again using Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11008; also available in Cat. No. A11053) (right panel). Note the significant signal enhancement with each amplification step.

Chromosome spreads were prepared from the cultured fibroblast cell line MRC-5 and hybridized with an a-satellite probe labeled with the Oregon Green® 488 dye and specific for chromosome 17. The probe was labeled using the ULYSIS Alexa Fluor® 488 Nucleic Acid Labeling Kit (Cat. No. U21659) (left panel). The signal was amplified using the Alexa Fluor® 488 conjugate of rabbit anti-fluorescein/Oregon Green® antibody (Cat. No. A11090; also available in Cat. No. A11053) (middle panel) and amplified once again using Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11008; also available in Cat. No. A11053) (right panel). Note the significant signal enhancement with each amplification step.

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