Amplification of FISH signals using the Alexa Fluor® 488 Signal-Amplification Kit for Fluorescein- and Oregon Green® Dye–Conjugated Probes.
Chromosome spreads were prepared from the cultured fibroblast cell line MRC-5 and hybridized with an a-satellite probe labeled with the Oregon Green® 488 dye and specific for chromosome 17. The probe was labeled using the ULYSIS Alexa Fluor® 488 Nucleic Acid Labeling Kit (Cat. No. U21659) (left panel). The signal was amplified using the Alexa Fluor® 488 conjugate of rabbit anti-fluorescein/Oregon Green® antibody (Cat. No. A11090; also available in Cat. No. A11053) (middle panel) and amplified once again using Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11008; also available in Cat. No. A11053) (right panel). Note the significant signal enhancement with each amplification step.
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›
Endothelial cells. Go ›
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›
Developing Drosophila embryo Go ›
Simultaneous detection of expression of five genes in a whole mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes Go ›
Labeled paint probes hybridized to human metaphase chromosomes Go ›