Simultaneous detection of three gene targets in a whole-mount Drosophila embryo by fluorescence in situ hybridization.

Pseudocolored green-fluorescent labeling represents a fluorescein-labeled cRNA probe detected using a rabbit anti-fluorescein/Oregon Green® primary antibody (Cat. No. A889) and an Alexa Fluor® 488 dye–labeled anti-rabbit secondary antibody (Cat. No. A11008). Pseudocolored yellow- and red-fluorescent labeling represents a biotinylated cRNA probe detected using HRP–streptavidin and Alexa Fluor® 568 tyramide (TSA Kit #24, Cat. No. T20934). Pseudocolored blue-fluorescent labeling represents a digoxigenin-labeled cRNA probe detected using a mouse anti-digoxigenin primary antibody in conjunction with an Alexa Fluor® 647 dye–labeled anti-mouse secondary antibody (Cat. No. A21235). The image was contributed by Ethan Bier and David Kosman, University of California, San Diego.

Pseudocolored green-fluorescent labeling represents a fluorescein-labeled cRNA probe detected using a rabbit anti-fluorescein/Oregon Green® primary antibody (Cat. No. A889) and an Alexa Fluor® 488 dye–labeled anti-rabbit secondary antibody (Cat. No. A11008). Pseudocolored yellow- and red-fluorescent labeling represents a biotinylated cRNA probe detected using HRP–streptavidin and Alexa Fluor® 568 tyramide (TSA Kit #24, Cat. No. T20934). Pseudocolored blue-fluorescent labeling represents a digoxigenin-labeled cRNA probe detected using a mouse anti-digoxigenin primary antibody in conjunction with an Alexa Fluor® 647 dye–labeled anti-mouse secondary antibody (Cat. No. A21235). The image was contributed by Ethan Bier and David Kosman, University of California, San Diego.

Related Products

Sku Name Size Price Qty
A11008 Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) Antibody 0.5 mL USD 149.00
A21235 Alexa Fluor® 647 Goat Anti-Mouse IgG (H+L) 0.5 mL USD 149.00
A889 Fluorescein⁄Oregon Green® Rabbit IgG Antibody Fraction 0.5 mL USD 289.00
T20934 TSA™ Kit #24, with HRP—Streptavidin and Alexa Fluor® 568 Tyramide 1 kit USD 471.00

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