Higher protein yields achieved with the MagicMedia™ E. coli Expression Medium
Eight different human ORFs were cloned into the Champion™ pET300/NT-DEST vector using Gateway® cloning. Positive clones were transformed into BL21(DE3) E. coli. The clones were expressed in LB + IPTG (lane 1), ready-to-use liquid MagicMedia™ medium (lane 2), or MagicMedia™ medium prepared from powder (lane 3). Two hundred microliters of each culture were lysed and analyzed on a Coomassie-stained NuPAGE® Novex® 4-12% Bis-Tris Protein Gel. M: SeeBlue® Protein Standard.
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›