Higher protein yields achieved with the MagicMedia™ E. coli Expression Medium

Eight different human ORFs were cloned into the Champion™ pET300/NT-DEST vector using Gateway® cloning. Positive clones were transformed into BL21(DE3) E. coli. The clones were expressed in LB + IPTG (lane 1), ready-to-use liquid MagicMedia™ medium (lane 2), or MagicMedia™ medium prepared from powder (lane 3). Two hundred microliters of each culture were lysed and analyzed on a Coomassie-stained NuPAGE® Novex® 4-12% Bis-Tris Protein Gel. M: SeeBlue® Protein Standard.

Eight different human ORFs were cloned into the Champion™ pET300/NT-DEST vector using Gateway® cloning. Positive clones were transformed into BL21(DE3) <i>E. coli</i>. The clones were expressed in LB + IPTG (lane 1), ready-to-use liquid MagicMedia™ medium (lane 2), or MagicMedia™ medium prepared from powder (lane 3).  Two hundred microliters of each culture were lysed and analyzed on a Coomassie-stained NuPAGE® Novex® 4-12% Bis-Tris Protein Gel. M: SeeBlue® Protein Standard.

Related Products

Sku Name Size Price Qty
K6803 MagicMedia™ E. coli Expression Medium 1 L USD 91.25
K6810 MagicMedia™ E. coli Expression Medium, Powder 1 L USD 46.19
K6815 MagicMedia™ E. coli Expression Medium, Powder 5 x 1 L USD 223.00

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