Amplification obtained with the Alexa Fluor® 488 Signal Amplification Kit for Fluorescein- and Oregon Green® Dye–Conjugated Probes.

Bovine pulmonary artery endothelial cells were labeled with anti–a-tubulin antibody (Cat. No. A11126) in combination with fluorescein goat anti–mouse IgG antibody (Cat. No. F2761) (left panel). The center panel shows the cells after treatment with Alexa Fluor® 488 rabbit anti-fluorescein/Oregon Green® antibody (Cat. No. A11090), and the right panel show the cells after additional labeling with the Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11008). The images were acquired using identical exposure times, and a bandpass filter set appropriate for fluorescein.

Bovine pulmonary artery endothelial cells were labeled with anti–a-tubulin antibody (Cat. No. A11126) in combination with fluorescein goat anti–mouse IgG antibody (Cat. No. F2761) (left panel). The center panel shows the cells after treatment with Alexa Fluor® 488 rabbit anti-fluorescein/Oregon Green® antibody (Cat. No. A11090), and the right panel show the cells after additional labeling with the Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11008). The images were acquired using identical exposure times, and a bandpass filter set appropriate for fluorescein.

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