Rhizoid cells from the marine alga Fucus serratus were pressure-microinjected with 10,000 MW Calcium Green™-1 dextran and 10,000 MW Texas Red® dextran.
Rhizoid cells from the marine alga Fucus serratus were pressure-microinjected with Ca2+-sensitive 10,000 MW Calcium Green™-1 dextran (Cat. no. C3713) and Ca2+-insensitive 10,000 MW Texas Red® dextran (D1828). Patterns of Ca2+ elevation following hypoosmotic treatment in dividing cells were visualized by confocal ratio imaging of Calcium Green™-1 (excited at 488 nm) and Texas Red® (excited at 568 nm). Emission ratio values were pseudocolored to represent calcium concentrations according to the scale bar in the lower left-hand corner. A) Sequential ratio images during the onset of a hypoosmotically induced (100% seawater to 50% seawater) Ca2+ wave shows an initial elevation of Ca2+ in the rhizoid apex, which declines before the onset of Ca2+ elevation arising in the apical nucleus (n1) region. B) A variation in this pattern was evident in a minority of cells where Ca2+ elevations were observed to arise in the subapical nucleus (n2) simultaneously with the apical Ca2+ elevation. The image was contributed by Colin Brownlee, Marine Biological Association of the United Kingdom, Plymouth, UK, and reproduced with permission from Proc Natl Acad Sci U S A 97, 1932 (2000).
Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran. Go ›
Secondary motor neurons in a male western mosquitofish (Gambusia affinis affinis) spinal cord. Go ›
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
Pseudocolored images of changes in intracellular free Ca2+ in AtT-20/D16v-F2 cells, monitored at 9 sec intervals with fluo-4, AM (F14201, F14202, F14217, F23917). Go ›
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›