RNA products from two transcription systems:
1 kb, 4 kb, or 9 kb templates were used in the MEGAscript™ Kit or in another manufacturer's in-vitro transcription kit according to protocol. A portion of each reaction (5 µL) was loaded onto a 1% formaldehyde gel and stained with ethidium bromide.
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›