ArrayScript™ Reverse Transcriptase produces up to twice the aRNA yield in MessageAMP™ II:
A comparison of ArrayScript™ (200 U), SuperScript® II (200 U), wild type MMLV-RT (200 U), and wild type AMV-RT (10 U) in MessageAMP™ II conditions demonstrate that ArrayScript™ outperforms other commercial RT's. 90 ng HeLa-S3 total RNA (28S/18S ratio = 1.7) was heat denatured in the presence of T7 Oligo (dT) at 70°C for 10 min. Reverse transcription was initiated with the RT enzyme and incubated at 42°C for 2 hr in an air incubator. The final aRNA yield was quantified by absorbance at 260 nm using a NanoDrop® spectrophotometer.
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›