False-color image of free Ca2+ concentration in a Purkinje neuron from embryonic mouse cerebellum.
False-color image of free Ca2+ concentration in a Purkinje neuron from embryonic mouse cerebellum. Neurons were grown in dispersed tissue culture for 12 days, loaded with the pentapotassium salt of fura-2 (Cat. no. F1200) using a microelectrode and then challenged with trans-ACPD, an agonist of metabotropic glutamate receptors, in the absence of extracellular Ca2+. The composite image, which represents the ratio of images obtained with excitation at 340 nm and 380 nm, reveals the mobilization of internal Ca2+ stores without contribution from Ca2+ influx. The image was contributed by D.J. Linden, Johns Hopkins University, and M. Smeyne and J.A. Connor, Roche Institute of Molecular Biology.
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
Pseudocolored images of changes in intracellular free Ca2+ in AtT-20/D16v-F2 cells, monitored at 9 sec intervals with fluo-4, AM (F14201, F14202, F14217, F23917). Go ›
Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran. Go ›
Phagocytosis in MMM Cells using pHrodo™ Red E. coli BioParticles® Conjugate® (Catalog # P35361) Go ›
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›
Developing Drosophila embryo Go ›
Cytoskeleton of a mixed population of granule neurons and glial cells Go ›