Fibroblast and neonatal cardiomyocyte. BODIPY FL phallacidin and Texas Red goat anti–mouse IgG (H+L) antibody.
Actin labeled with BODIPY FL phallacidin (B607) and vinculin, a cytoskeletal focal adhesion protein, tagged with a monoclonal anti-vinculin antibody that was subsequently probed with Texas Red goat anti–mouse IgG antibody (T862). The large triangular cell is a fibroblast containing green actin stress fibers terminating in red focal adhesions. The neighboring polygonal cell, a rat neonatal cardiomyocyte, contains green striated actin in the myofibrils terminating in the focal adhesions. The close apposition of the two stains results in a yellowish-orange color. Image contributed by Mark B. Snuggs and W. Barry VanWinkle, University of Texas, Houston.