Bovine pulmonary artery endothelial cells. Anti–dinitrophenyl-KLH antibody and LIVE/DEAD® Reduced Biohazard Viability/Cytotoxicity Kit.
A mixed population of trinitrophenyl-sensitized and nonsensitized bovine pulmonary artery endothelial cells that have been probed with anti–dinitrophenyl-KLH antibody (Cat. no. A6430), incubated with rabbit complement and then stained using the LIVE/DEAD® Reduced Biohazard Viability/Cytotoxicity Kit #1 (Cat. no. L7013). With this kit, the membrane-permeant SYTO® 10 dye labels the nucleic acids of live cells with green fluorescence, and the membrane-impermeant DEAD Red™ dye labels nucleic acids of membrane-compromised cells with red fluorescence. Subsequent aldehyde-based fixation inactivates pathogens without distorting the original staining pattern. This multiple-exposure image was acquired with bandpass optical filters appropriate for fluorescein and Texas Red® dyes.