Adherent 3T3 fibroblasts. Texas Red 3000 MW dextran and Calcium Green-1 70,000 MW dextran.
Adherent 3T3 fibroblasts labeled with 3000 MW Texas Red dextran (D3329) and 70,000 MW Calcium Green-1 dextran (C3714). Both dextrans were loaded into the fibroblasts using the Influx pinocytic cell-loading reagent (I14402). The images were acquired using a fluorescence microscope equipped with a cooled CCD camera and bandpass filter sets appropriate for either the Texas Red dye (left image) or fluorescein (right image). The center image represents a composite of the cells labeled with both dextrans. The 3000 MW Texas Red dextran is distributed throughout the cell, but the 70,000 MW Calcium Green-1 dextran is excluded from the nucleus by the nuclear membrane.
Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran. Go ›
Rhizoid cells from the marine alga Fucus serratus were pressure-microinjected with 10,000 MW Calcium Green™-1 dextran and 10,000 MW Texas Red® dextran. Go ›
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
Pseudocolored images of changes in intracellular free Ca2+ in AtT-20/D16v-F2 cells, monitored at 9 sec intervals with fluo-4, AM (F14201, F14202, F14217, F23917). Go ›
Live CRE BAG 2 cells. Go ›
lacZ-negative (NIH 3T3) and lacZ-positive (CRE BAG 2) cells. Go ›
Table 2 - Tris-Glycine/Tricine to NuPAGE® Gel conversion chart Go ›
Table 1 - Migration patterns of protein standards* on NuPAGE® Gels Go ›