Sporulating Bacillus subtilis.
The morphology of sporulating Bacillus subtilis in the early stages of forespore engulfment. The membranes and chromosomes of both the forespore and the larger mother cell are stained with FM 4-64 (red; Cat. No. T3166, T13320) and DAPI (blue, Cat. No. D1306, D3571, D21490), respectively. The small green-fluorescent patch indicates the localization of a GFP fusion to SPoIIIE, a protein essential for translocation of the forespore chromosome that may also regulate membrane fusion events (see Proc Natl Acad Sci U S A (1999) 96:14553). The background contains sporangia at various stages in the engulfment process stained with MitoTracker Green FM (green, Cat No. M7514) and FM 4-64 (red).