Development of the leech central nervous system into segments characterized with a 10,000 MW fluorescein dextran.
Development of the leech central nervous system into segments was characterized with the cell lineage tracer lysine-fixable 10,000 MW fluorescein dextran (fluoro-emerald, Cat. no. D1820). Fluoro-emerald was injected into a neuroectodermal cell of a leech embryo. The teloblast eventually differentiated into five segmentally iterated ganglia labeled with fluoro-emerald (pseudocolored blue in this image). Associated muscle fibers were identified with a Lan 3-14 monoclonal antibody and visualized with a Cy3 antibody (pseudocolored red). Nuclei were counterstained with TOTO®-3 dye (Cat. no. T3604, pseudocolored green). Image reprinted from the cover of Development 127 (4) (2000), used with the permission of The Company of Biologists Ltd.