Human metaphase chromosome spreads.

Fluorescent probes generated with ARES DNA Labeling Kits hybridized to human metaphase chromosome spreads. Centromere probes specific for chromosomes 17, 1 and 15 were prepared by nick translation and labeled with kits containing green-fluorescent Alexa Fluor 488 (A21665), red-orange–fluorescent Alexa Fluor 546 (A21667) and red-fluorescent Alexa Fluor 594 (A21669) dyes. DNA was counterstained with the blue-fluorescent Hoechst 33342 dye (H1399, H3570, H21491), and the slides were mounted using the ProLong Antifade Kit (P7481). This multiple-exposure image was obtained with bandpass filter sets appropriate for fluorescein, rhodamine, Texas Red dye and DAPI.

Fluorescent probes generated with ARES DNA Labeling Kits hybridized to human metaphase chromosome spreads. Centromere probes specific for chromosomes 17, 1 and 15 were prepared by nick translation and labeled with kits containing green-fluorescent Alexa Fluor 488 (A21665), red-orange–fluorescent Alexa Fluor 546 (A21667) and red-fluorescent Alexa Fluor 594 (A21669) dyes. DNA was counterstained with the blue-fluorescent Hoechst 33342 dye (H1399, H3570, H21491), and the slides were mounted using the ProLong Antifade Kit (P7481). This multiple-exposure image was obtained with bandpass filter sets appropriate for fluorescein, rhodamine, Texas Red dye and DAPI.

Related Products

Sku Name Size Price Qty
A21665 ARES™ Alexa Fluor® 488 DNA Labeling Kit 1 kit USD 362.00
A21667 ARES™ Alexa Fluor® 546 DNA Labeling Kit 1 kit USD 362.00
A21669 ARES™ Alexa Fluor® 594 DNA Labeling Kit 1 kit USD 362.00
P7481 ProLong® Antifade Kit 1 kit USD 241.00
H1399 Hoechst 33342, Trihydrochloride, Trihydrate, 100 mg 100 mg USD 63.25
H21491 Hoechst 33258, Pentahydrate (bis-Benzimide) - FluoroPure™ Grade 100 mg USD 74.00
H3570 Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg⁄mL Solution in Water 10 mL USD 84.00

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