Detection of an oligohistidine fusion protein with the Penta·His mouse monoclonal antibody.

Detection of an oligohistidine fusion protein with the Penta·His mouse monoclonal antibody. Twofold serial dilutions of an Escherichia coli lysate containing overexpressed oligomycin sensitivity–conferring protein (OSCP) fused with a hexahistidine domain were run an SDS-polyacrylamide gel and blotted onto a PVDF membrane. The blot was stained with the SYPRO Ruby protein blot stain (S11791) to detect the entire protein profile (top). After imaging, the blot was incubated with Penta·His mouse IgG1 monoclonal antibody (P21315), followed by immunodetection using an alkaline phosphatase conjugate of goat anti–mouse antibody in conjunction with DDAO phosphate (bottom).

Detection of an oligohistidine fusion protein with the Penta·His mouse monoclonal antibody. Twofold serial dilutions of an <i>Escherichia coli</i> lysate containing overexpressed oligomycin sensitivity–conferring protein (OSCP) fused with a hexahistidine domain were run an SDS-polyacrylamide gel and blotted onto a PVDF membrane. The blot was stained with the SYPRO Ruby protein blot stain (S11791) to detect the entire protein profile (top). After imaging, the blot was incubated with Penta·His mouse IgG<sub>1</sub> monoclonal antibody (P21315), followed by immunodetection using an alkaline phosphatase conjugate of goat anti–mouse antibody in conjunction with DDAO phosphate (bottom).

Related Products

Sku Name Size Price Qty
P21315 Penta•His™ Mouse IgG1, Monoclonal Antibody (clone Anti-Pentahistidine), BSA-free 100 µg USD 626.00
S11791 SYPRO® Ruby Protein Blot Stain 200 mL USD 166.00

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