Detection of an oligohistidine fusion protein with the Penta·His mouse monoclonal antibody.
Detection of an oligohistidine fusion protein with the Penta·His mouse monoclonal antibody. Twofold serial dilutions of an Escherichia coli lysate containing overexpressed oligomycin sensitivity–conferring protein (OSCP) fused with a hexahistidine domain were run an SDS-polyacrylamide gel and blotted onto a PVDF membrane. The blot was stained with the SYPRO Ruby protein blot stain (S11791) to detect the entire protein profile (top). After imaging, the blot was incubated with Penta·His mouse IgG1 monoclonal antibody (P21315), followed by immunodetection using an alkaline phosphatase conjugate of goat anti–mouse antibody in conjunction with DDAO phosphate (bottom).
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain. Go ›
2D gel stained with the SYPRO Ruby protein gel stain and the Pro-Q Emerald 300 reagent. Go ›
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›
Western blot analysis of (A) MDA-MB-231, (B) MDA-MB-468, (C) TF-1, (D) K562, (E) Caco-2, and (F) PC-3 cell lysates, and (G) mouse testis homogenates using our Rb anti-Metadherin (C-term) (Cat. No. 40-6400). Go ›
Multicolor fluorescence analysis of muntjac fibroblasts. Go ›