J774 mouse macrophage cell.

A J774 mouse macrophage cell sequentially stained with BODIPY FL ganglioside GM1 (B13950) and then with Alexa Fluor 555 dye–labeled cholera toxin subunit B (Cat. No. C22843, C34776; also available as a component of Cat. No. V34404). The cell was then treated with an anti–CT-B antibody (a component of Cat. No. V34404) to induce crosslinking. Alexa Fluor 555 dye fluorescence (top panel, red) and BODIPY FL dye fluorescence (middle panel, green) were imaged separately and overlaid to emphasize the coincident staining (bottom panel, yellow). Nuclei were stained with blue-fluorescent Hoechst 33342 (Cat. No. H1399, H3570, H21492).

A J774 mouse macrophage cell sequentially stained with BODIPY FL ganglioside G<sub>M1</sub> (B13950) and then with Alexa Fluor 555 dye–labeled cholera toxin subunit B (Cat. No. C22843, C34776; also available as a component of Cat. No. V34404). The cell was then treated with an anti–CT-B antibody (a component of Cat. No. V34404) to induce crosslinking. Alexa Fluor 555 dye fluorescence (top panel, red) and BODIPY FL dye fluorescence (middle panel, green) were imaged separately and overlaid to emphasize the coincident staining (bottom panel, yellow). Nuclei were stained with blue-fluorescent Hoechst 33342 (Cat. No. H1399, H3570, H21492).

Related Products

Sku Name Size Price Qty
B13950 BODIPY® FL C5-Ganglioside GM1 25 µg USD 239.00
C22843 Cholera Toxin Subunit B (Recombinant), Alexa Fluor® 555 Conjugate 500 µg USD 548.00
C34776 Cholera Toxin Subunit B (Recombinant), Alexa Fluor® 555 Conjugate 100 µg USD 188.00
H1399 Hoechst 33342, Trihydrochloride, Trihydrate, 100 mg 100 mg USD 63.25
H21492 Hoechst 33342, Trihydrochloride, Trihydrate - FluoroPure™ Grade 100 mg USD 75.00
H3570 Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg⁄mL Solution in Water 10 mL USD 84.00
V34404 Vybrant® Alexa Fluor® 555 Lipid Raft Labeling Kit 1 kit USD 462.00

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