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SimpliAmp™ Thermal Cycler

The SimpliAmp™ PCR machine can be used with a 4°C hold. Further, our in-house QC testing requires the passing of a “no leak” test make sure that any accumulated water (due to condensation when the block temperature is set to low temperature for a long period of time) will not leak into the instrument causing electronic failure.

The storage capacity for the SimpliAmp™ Thermal Cycler is ~5.9 GB. The run methods and run records stored use most of the memory. It can store 1.2 million run methods.

The two different types of trays are for use with different tubes:

  • 4381850, MicroAmp 96-Well Tray/Retainer Set – this Tray is for use with Single Tubes and/or Strip Tubes.
  • 4379983, MicroAmp 96-Well Tray – this Tray is for use with Single Tube with Cap and/or Strip Tubes with Caps.

We offer only a 96-well standard (0.2 mL) block that is not interchangeable.

The compatible plates and plastics are the same as those used with the ProFlex™ 96-Well PCR System and can be viewed here.

The maximum block ramp rate of the SimpliAmp™ PCR machine is 4°C/sec.

The Auto Delta feature allows you to incrementally increase or decrease the temperature each cycle at a step within a cycling stage. One common application for this is touchdown PCR.

The SimpliAmp™ thermocycler features innovative VeriFlex™ Blocks for enhanced PCR functionality. Three separate Peltier blocks (4 columns each) provide maximal versatility and flexibility and offer two key benefits:

  • PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
  • Run more experiments: the three Peltier blocks can also be used to run up to three different annealing temperatures in the same run.

To add an infinite hold, select the infinity (∞) symbol for program.

Like the ProFlex™ PCR System, the SimpliAmp™ Thermal Cycler can be connected wirelessly with an optional USB dongle, Cat. No. 4483658.

Proflex™ PCR System

The ProFlex™ PCR machine has 4 different blocks that can be changed with the flip of a switch, including the first-of-its-kind 3 x 32-well independent block. This block allows up to 3 experiments to be run simultaneously, completely independently of each other and without interference. A dual 96-well block is available for your high throughput sequencing needs and a dual flat block is available to support OpenArray® plate technology for genotyping analysis on the QuantStudio™ 12K Flex Real-Time PCR System.

The plates and plastics will be specific to the block type. Please refer to the tables here for a list of compatible plastics, based on your block type.

The 96-well and 3 x 32-well system have a max block ramp rate of 6.0°C/sec and the dual 96-well and dual flat blocks have a max block ramp rate of 2.0°C/sec.

The Auto Delta feature allows you to incrementally increase or decrease the temperature each cycle at a step within a cycling stage. One common application for this is touchdown PCR.

In the ProFlex™ PCR thermocycler, the 96-well and 3 x 32-well blocks feature innovative VeriFlex™ Blocks for enhanced PCR functionality. Six separate Peltier blocks (2 on each 32-well block for 3 x 32-well) provide maximal versatility and flexibility and offer two key benefits:

  1. PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
  2. Run more experiments: the six Peltier blocks can also be used to run up to six different annealing temperatures in the same run.

To add an infinite hold, select the infinity () symbol for program.

Veriti® Thermal Cycler

The plates and plastics will be specific to the block type. Choose the appropriate block type below for a list of the compatible tubes, plates, and films.

Veriti® Thermal Cycler
Block Type
Max Block Ramp Rate
(°C/sec)
96-Well 3.9
96-Well Fast 5.0
384-Well 3.7
60-Well 3.3

The Auto Delta feature allows you to incrementally increase or decrease the temperature each cycle at a step within a cycling stage. One common application for this is touchdown PCR.

The Veriti® thermocycler with a 96-well block (Fast and Standard) features innovative VeriFlex™ Blocks for enhanced PCR functionality. Six separate Peltier blocks provide maximal versatility and flexibility and offer two key benefits:

  1. PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
  2. Run more experiments: the six Peltier blocks can also be used to run up to six different annealing temperatures in the same run.

To add an infinite hold, select the infinity () symbol for program.

2720 Thermal Cycler

The 2720 Thermal Cycler contains a 96-well standard block, which can be used with 0.2 mL tubes and plates. For more options in block compatibility, you can review our other thermal cyclers here.

The max block ramp rate of the 2720 PCR machine is 2.3°C/sec.

The Auto Delta feature allows you to incrementally increase or decrease the temperature each cycle at a step within a cycling stage. One common application for this is touchdown PCR.

No, the 2720 PCR machine does not have a gradient feature. Our newer thermal cyclers including the ProFlex™ PCR System and Veriti® Thermal Cycler have better-than-gradient VeriFlex™ blocks.

To add an infinite hold, set the time for the hold to ‘99.59’. The screen should then default to the infinity () symbol for your hold.

GeneAmp® PCR System 9700

The GeneAmp® PCR System 9700 offers interchangeable sample blocks in a variety of well formats and materials to accommodate a wide range of applications.

The plates and plastics will be specific to the block type. Please refer to the tables here for a list of compatible plastics, based on your block type.

The Auto Delta feature allows you to incrementally increase or decrease the temperature each cycle at a step within a cycling stage. One common application for this is touchdown PCR.

No, the GeneAmp® PCR System 9700 does not have a gradient feature. Our newer thermal cyclers including the ProFlex™ PCR System and Veriti® Thermal Cycler have better-than-gradient VeriFlex™ Blocks.

To add an infinite hold, set the time for the hold to ‘99.59’. The screen should then default to the infinity () symbol for your hold.

Please refer to the guidelines in this protocol specific to the GeneAmp® PCR System 9700 and block type.

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