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Instrument

For troubleshooting guides, please register for our QuantStudio™ 3D Digital PCR System User Group Forum in the Applied Biosystems community.

Please follow the procedures described in "Chapter A: Maintenance" of the QuantStudio™ 3D Digital PCR System User Guide to replace the fuses. Make sure to only replace with those listed and certified fuses of the same type and rating as those currently in the QuantStudio™ 3D instrument.

Please follow the procedures starting in "Chapter A: Maintenance" of the QuantStudio™ 3D Digital PCR System User Guide to update the instrument firmware.

AnalysisSuite™ software is optimized for Google Chrome v4.0 or later. Firefox also works, but Internet Explorer is not recommended.

Data Analysis

First you need to know the amount of nanograms per copy of your sample. In this example, we’ll use human genomic DNA as the source of DNA.

Now you just need to multiply ng/copy by copies/µL. You have 2,500 copies/µL of human gDNA.

     2,500 copies/µL x 0.0033 ng/copy = 8.25 ng/copy

If you include all of the necessary dilution factors into the AnalysisSuite™ software, the software will give you the copies/µL in your stock. There are 2 dilutions you need to take into account. The first is the dilution of the sample in the reaction. The second is the dilution of the stock that you make before adding it to the digital PCR reaction.

For example, say you want to add 1 µL of a sample that has been diluted 1:10 from the stock. If you add 1 µL of your sample to a 16 µL (final volume) reaction, the dilution factor of the sample is 1:16 or 1/16 = 0.0625. Since the stock has also been diluted 1:10 (0.1), you also need to factor this in. The final dilution factor to enter into the software is 0.0625 x 0.1 = 0.00625 (1:160). As you can see below, you can use either annotation to indicate the dilution factor in the AnalysisSuite™ software. If you enter that value into the “Dilution” column, the software will give you the copies/µL in your starting material (stock).